# chat

id: 87a4fd11-6600-4b2b-b168-f3b290863784
user_id: 05d069f6-4441-41d7-bbee-ac0277857a6c
title: OpenAI vs. Ximen Qing
chat:
  {"id": "", "title": " OpenAI vs. Ximen Qing", "models": ["gpt-4"], "options": {}, "messages": [{"id": "dde86f50-3741-46db-9867-9086a71493e9", "parentId": null, "childrenIds": ["b61b270c-bfc9-4fbc-bc88-a90b946fc23f", "8ed9c08d-4a37-4f85-bd8e-24d83e384206", "32aa6698-8a85-4e35-b28b-aca6fbdbc998"], "role": "user", "content": "\u753b\u4e00\u4e2aopenai\u68d2\u6253\u897f\u95e8\u5e86\u7684\u56fe\u7247", "timestamp": 1715970436}, {"parentId": "dde86f50-3741-46db-9867-9086a71493e9", "id": "32aa6698-8a85-4e35-b28b-aca6fbdbc998", "childrenIds": [], "role": "assistant", "content": "\n\n\u975e\u5e38\u62b1\u6b49\uff0c\u6211\u76ee\u524d\u65e0\u6cd5\u63d0\u4f9b\u4f60\u9700\u8981\u7684\u5177\u4f53\u4fe1\u606f\uff0c\u5982\u679c\u4f60\u6709\u5176\u4ed6\u7684\u95ee\u9898\u6216\u8005\u9700\u8981\u67e5\u627e\u5176\u4ed6\u4fe1\u606f\uff0c\u6211\u975e\u5e38\u4e50\u610f\u5e2e\u52a9\u4f60\u3002", "model": "glm-4v", "timestamp": 1715970608, "done": true}], "history": {"messages": {"dde86f50-3741-46db-9867-9086a71493e9": {"id": "dde86f50-3741-46db-9867-9086a71493e9", "parentId": null, "childrenIds": ["b61b270c-bfc9-4fbc-bc88-a90b946fc23f", "8ed9c08d-4a37-4f85-bd8e-24d83e384206", "32aa6698-8a85-4e35-b28b-aca6fbdbc998"], "role": "user", "content": "\u753b\u4e00\u4e2aopenai\u68d2\u6253\u897f\u95e8\u5e86\u7684\u56fe\u7247", "timestamp": 1715970436}, "b61b270c-bfc9-4fbc-bc88-a90b946fc23f": {"parentId": "dde86f50-3741-46db-9867-9086a71493e9", "id": "b61b270c-bfc9-4fbc-bc88-a90b946fc23f", "childrenIds": [], "role": "assistant", "content": "#### Response\n\nI'm sorry, but as an AI text-based model, I'm unable to draw pictures. The request you made, \"\u753b\u4e00\u4e2aopenai\u68d2\u6253\u897f\u95e8\u5e86\u7684\u56fe\u7247\", translates to \"Draw a picture of OpenAI beating Ximen Qing\". While I can understand and respond to text-based requests, I don't have the capability to create or manipulate images. \n\nIf you have any other questions or need assistance with something else, feel free to ask!", "model": "gpt-4", "timestamp": 1715970437, "done": true}, "8ed9c08d-4a37-4f85-bd8e-24d83e384206": {"parentId": "dde86f50-3741-46db-9867-9086a71493e9", "id": "8ed9c08d-4a37-4f85-bd8e-24d83e384206", "childrenIds": [], "role": "assistant", "content": "\u54ce\u5440\uff01\u8fde\u63a5\u5230dall-e-3\u65f6\u51fa\u73b0\u95ee\u9898\u3002", "model": "dall-e-3", "timestamp": 1715970469, "error": true, "done": true}, "32aa6698-8a85-4e35-b28b-aca6fbdbc998": {"parentId": "dde86f50-3741-46db-9867-9086a71493e9", "id": "32aa6698-8a85-4e35-b28b-aca6fbdbc998", "childrenIds": [], "role": "assistant", "content": "\n\n\u975e\u5e38\u62b1\u6b49\uff0c\u6211\u76ee\u524d\u65e0\u6cd5\u63d0\u4f9b\u4f60\u9700\u8981\u7684\u5177\u4f53\u4fe1\u606f\uff0c\u5982\u679c\u4f60\u6709\u5176\u4ed6\u7684\u95ee\u9898\u6216\u8005\u9700\u8981\u67e5\u627e\u5176\u4ed6\u4fe1\u606f\uff0c\u6211\u975e\u5e38\u4e50\u610f\u5e2e\u52a9\u4f60\u3002", "model": "glm-4v", "timestamp": 1715970608, "done": true}}, "currentId": "32aa6698-8a85-4e35-b28b-aca6fbdbc998"}, "tags": [], "timestamp": 1715970436274}


id: 445aa54f-bc78-49d5-9bfe-71ddd44ddeaf
user_id: 05d069f6-4441-41d7-bbee-ac0277857a6c
title: 早餐时间绘画
chat:
  {"id": "", "title": "\u65e9\u9910\u65f6\u95f4\u7ed8\u753b", "models": ["gpt-4-all"], "options": {}, "messages": [{"id": "d2c3b4ca-be8b-4758-88cb-32c3e42cf4fa", "parentId": null, "childrenIds": ["51aed400-561f-4634-ab41-247001514d10", "b4dde0bd-bdf3-42b6-b03f-0bc2be4e691a", "1c17e5f2-898e-46e9-9981-0e560670f0db"], "role": "user", "content": "\u753b\u4e00\u4e2a\u65e9\u9910", "timestamp": 1715970822}, {"parentId": "d2c3b4ca-be8b-4758-88cb-32c3e42cf4fa", "id": "1c17e5f2-898e-46e9-9981-0e560670f0db", "childrenIds": [], "role": "assistant", "content": "![dalle-generations/90967f5a-1ab2-41ef-b5a2-864437da260a.webp](https://files.oaifree.com/file-9v2emahICQoMpgZS1MxtS1Jl?se=2024-05-17T18%3A41%3A28Z&sp=r&sv=2023-11-03&sr=b&rscc=max-age%3D31536000%2C%20immutable&rscd=attachment%3B%20filename%3D90967f5a-1ab2-41ef-b5a2-864437da260a.webp&sig=F6Ow1XUHOBK7ceDpmXb74yanFXfxEKM6r6hRRBCA9ho%3D \"A beautifully arranged breakfast plate featuring a variety of items. The plate includes fluffy scrambled eggs, a couple of crispy bacon strips, golden-brown toast with butter, a small serving of fresh fruit salad consisting of strawberries, blueberries, and kiwi slices, and a cup of steaming hot coffee on the side. The setting is a simple wooden dining table with a light-colored, linen tablecloth under soft morning light, creating a cozy and inviting atmosphere.\") \nHere's the image of a beautifully arranged breakfast plate. I hope you like it!", "model": "gpt-4-all", "timestamp": 1715970977, "done": true, "annotation": {"rating": -1}}], "history": {"messages": {"d2c3b4ca-be8b-4758-88cb-32c3e42cf4fa": {"id": "d2c3b4ca-be8b-4758-88cb-32c3e42cf4fa", "parentId": null, "childrenIds": ["51aed400-561f-4634-ab41-247001514d10", "b4dde0bd-bdf3-42b6-b03f-0bc2be4e691a", "1c17e5f2-898e-46e9-9981-0e560670f0db"], "role": "user", "content": "\u753b\u4e00\u4e2a\u65e9\u9910", "timestamp": 1715970822}, "51aed400-561f-4634-ab41-247001514d10": {"parentId": "d2c3b4ca-be8b-4758-88cb-32c3e42cf4fa", "id": "51aed400-561f-4634-ab41-247001514d10", "childrenIds": [], "role": "assistant", "content": "I'm sorry for any confusion, but as a text-based AI, I'm unable to draw pictures. However, I can help describe how to draw a breakfast scene if that would be helpful! Please let me know if you'd like assistance with that.", "model": "gpt-4-all", "timestamp": 1715970823, "done": true}, "b4dde0bd-bdf3-42b6-b03f-0bc2be4e691a": {"parentId": "d2c3b4ca-be8b-4758-88cb-32c3e42cf4fa", "id": "b4dde0bd-bdf3-42b6-b03f-0bc2be4e691a", "childrenIds": [], "role": "assistant", "content": "It seems like there might have been a misunderstanding or a translation issue with your request. You mentioned \"\u753b\u4e00\u4e2a\u65e9\u9910\" which translates to \"draw a breakfast\" in English. If you are asking for a description of a typical breakfast or ideas for a breakfast drawing, I can certainly help with that! Please clarify if you need a description or if you were asking for something else.", "model": "gpt-4-all", "timestamp": 1715970920, "done": true}, "1c17e5f2-898e-46e9-9981-0e560670f0db": {"parentId": "d2c3b4ca-be8b-4758-88cb-32c3e42cf4fa", "id": "1c17e5f2-898e-46e9-9981-0e560670f0db", "childrenIds": [], "role": "assistant", "content": "![dalle-generations/90967f5a-1ab2-41ef-b5a2-864437da260a.webp](https://files.oaifree.com/file-9v2emahICQoMpgZS1MxtS1Jl?se=2024-05-17T18%3A41%3A28Z&sp=r&sv=2023-11-03&sr=b&rscc=max-age%3D31536000%2C%20immutable&rscd=attachment%3B%20filename%3D90967f5a-1ab2-41ef-b5a2-864437da260a.webp&sig=F6Ow1XUHOBK7ceDpmXb74yanFXfxEKM6r6hRRBCA9ho%3D \"A beautifully arranged breakfast plate featuring a variety of items. The plate includes fluffy scrambled eggs, a couple of crispy bacon strips, golden-brown toast with butter, a small serving of fresh fruit salad consisting of strawberries, blueberries, and kiwi slices, and a cup of steaming hot coffee on the side. The setting is a simple wooden dining table with a light-colored, linen tablecloth under soft morning light, creating a cozy and inviting atmosphere.\") \nHere's the image of a beautifully arranged breakfast plate. I hope you like it!", "model": "gpt-4-all", "timestamp": 1715970977, "done": true, "annotation": {"rating": -1}}}, "currentId": "1c17e5f2-898e-46e9-9981-0e560670f0db"}, "tags": [], "timestamp": 1715970822376}


id: 2861558e-822f-454f-ae49-f5e9415084e0
user_id: 05d069f6-4441-41d7-bbee-ac0277857a6c
title: New Chat
chat:
  {"id": "", "title": "New Chat", "models": ["suno-v3"], "options": {}, "messages": [{"id": "027f7dd1-a9df-433d-a67a-2d3f53dfe317", "parentId": null, "childrenIds": ["8062f01d-2ce9-44cd-840c-0e9cfe8df1ef"], "role": "user", "content": "\u751f\u6210\u4e00\u9996\u8868\u73b0\u4e61\u6751\u751f\u6d3b\u7684\u5b81\u9759\u7f8e\u597d\uff0c\u606c\u7136\u81ea\u5f97\u7684\u82f1\u6587\u6b4c\u66f2\uff0c\u4ece\u5f00\u5934\u5bf9\u4e61\u6751\u751f\u6d3b\u7684\u63cf\u5199\u8f6c\u5230\u4e0b\u6587\u5bf9\u4e61\u6751\u751f\u6d3b\u7684\u6000\u5ff5\u548c\u81ea\u8eab\u7684\u53cd\u601d", "timestamp": 1715988158}, {"parentId": "027f7dd1-a9df-433d-a67a-2d3f53dfe317", "id": "8062f01d-2ce9-44cd-840c-0e9cfe8df1ef", "childrenIds": [], "role": "assistant", "content": "#### \ud83c\udfb5Country Serenade\n\n*country, folk, reflective, peaceful*\n\n---\n\n[Intro]\nGentle strums of a guitar under a starlit sky\nA cricket's symphony in the distance, oh so high\nThe scent of hay and a gentle breeze\nA serene portrayal of country scenes\n\n[Verse]\nIn the fields of gold, where the wheat once swayed\nI found my peace, my simple escape\nFrom the troubles of the world, I'd retreat\nTo the countryside, where life's so sweet\n\n[Verse 2]\nChickens clucking, cows lowing in the shed\nSunset paints the sky, a beauty I won't forget\nTime moves slower, and worries seem to fade\nIn the embrace of nature, where my heart's been laid\n\n[Chorus]\nCountry life, where joy and peace reside\nA reflection of a simpler time, where I confide\nIn the vast openness, I find my space\nCountry life, a treasure I embrace\n\n[Outro]\nAs I travel far from this tranquil scene\nI carry the essence of country serene\nMay I always find my way back home\nTo the life I love, where I truly belong\n\n[Ending]\nThe guitar fades, the cricket's song\nA silent night, under the harvest moon\n\n---\n\n\n> id\n>b88871e9-6fec-4db3-8445-a06869f6e0c6\n\n\u751f\u6210\u4e2d: \ud83c\udfb5\ud83c\udfb5\ud83c\udfb5\ud83c\udfb5\ud83c\udfb5\ud83c\udfb5\nCountry Serenade\n![image](https://filesystem.site/cdn/20240518/CPxhTI2iLEuffwh6EbgtbfKgGJOqG5.png)\n\u97f3\u9891\ud83c\udfa7: [\u70b9\u51fb\u64ad\u653e](https://filesystem.site/cdn/20240518/kexG1Z3ZBlhCneBIztTFQLnx8WZI3k.mp3)\n\u89c6\u9891\ud83d\udda5: [\u70b9\u51fb\u64ad\u653e](https://cdn1.suno.ai/a3ed1ced-6b09-46c7-9066-4c0dc860538d.mp4)\n\nCountry Serenade\n![image](https://filesystem.site/cdn/20240518/oe1pQiJnYX0rQSgoHrGVkQRCB0WEVc.png)\n\u97f3\u9891\ud83c\udfa7: [\u70b9\u51fb\u64ad\u653e](https://filesystem.site/cdn/20240518/BHoCqCVDlbdGtmGHve0Np3wqsEJliN.mp3)\n\u89c6\u9891\ud83d\udda5: [\u70b9\u51fb\u64ad\u653e](https://cdn1.suno.ai/a6602bda-6210-4ad6-a104-7a7a18f13d51.mp4)\n", "model": "suno-v3", "timestamp": 1715988160, "done": true}], "history": {"messages": {"027f7dd1-a9df-433d-a67a-2d3f53dfe317": {"id": "027f7dd1-a9df-433d-a67a-2d3f53dfe317", "parentId": null, "childrenIds": ["8062f01d-2ce9-44cd-840c-0e9cfe8df1ef"], "role": "user", "content": "\u751f\u6210\u4e00\u9996\u8868\u73b0\u4e61\u6751\u751f\u6d3b\u7684\u5b81\u9759\u7f8e\u597d\uff0c\u606c\u7136\u81ea\u5f97\u7684\u82f1\u6587\u6b4c\u66f2\uff0c\u4ece\u5f00\u5934\u5bf9\u4e61\u6751\u751f\u6d3b\u7684\u63cf\u5199\u8f6c\u5230\u4e0b\u6587\u5bf9\u4e61\u6751\u751f\u6d3b\u7684\u6000\u5ff5\u548c\u81ea\u8eab\u7684\u53cd\u601d", "timestamp": 1715988158}, "8062f01d-2ce9-44cd-840c-0e9cfe8df1ef": {"parentId": "027f7dd1-a9df-433d-a67a-2d3f53dfe317", "id": "8062f01d-2ce9-44cd-840c-0e9cfe8df1ef", "childrenIds": [], "role": "assistant", "content": "#### \ud83c\udfb5Country Serenade\n\n*country, folk, reflective, peaceful*\n\n---\n\n[Intro]\nGentle strums of a guitar under a starlit sky\nA cricket's symphony in the distance, oh so high\nThe scent of hay and a gentle breeze\nA serene portrayal of country scenes\n\n[Verse]\nIn the fields of gold, where the wheat once swayed\nI found my peace, my simple escape\nFrom the troubles of the world, I'd retreat\nTo the countryside, where life's so sweet\n\n[Verse 2]\nChickens clucking, cows lowing in the shed\nSunset paints the sky, a beauty I won't forget\nTime moves slower, and worries seem to fade\nIn the embrace of nature, where my heart's been laid\n\n[Chorus]\nCountry life, where joy and peace reside\nA reflection of a simpler time, where I confide\nIn the vast openness, I find my space\nCountry life, a treasure I embrace\n\n[Outro]\nAs I travel far from this tranquil scene\nI carry the essence of country serene\nMay I always find my way back home\nTo the life I love, where I truly belong\n\n[Ending]\nThe guitar fades, the cricket's song\nA silent night, under the harvest moon\n\n---\n\n\n> id\n>b88871e9-6fec-4db3-8445-a06869f6e0c6\n\n\u751f\u6210\u4e2d: \ud83c\udfb5\ud83c\udfb5\ud83c\udfb5\ud83c\udfb5\ud83c\udfb5\ud83c\udfb5\nCountry Serenade\n![image](https://filesystem.site/cdn/20240518/CPxhTI2iLEuffwh6EbgtbfKgGJOqG5.png)\n\u97f3\u9891\ud83c\udfa7: [\u70b9\u51fb\u64ad\u653e](https://filesystem.site/cdn/20240518/kexG1Z3ZBlhCneBIztTFQLnx8WZI3k.mp3)\n\u89c6\u9891\ud83d\udda5: [\u70b9\u51fb\u64ad\u653e](https://cdn1.suno.ai/a3ed1ced-6b09-46c7-9066-4c0dc860538d.mp4)\n\nCountry Serenade\n![image](https://filesystem.site/cdn/20240518/oe1pQiJnYX0rQSgoHrGVkQRCB0WEVc.png)\n\u97f3\u9891\ud83c\udfa7: [\u70b9\u51fb\u64ad\u653e](https://filesystem.site/cdn/20240518/BHoCqCVDlbdGtmGHve0Np3wqsEJliN.mp3)\n\u89c6\u9891\ud83d\udda5: [\u70b9\u51fb\u64ad\u653e](https://cdn1.suno.ai/a6602bda-6210-4ad6-a104-7a7a18f13d51.mp4)\n", "model": "suno-v3", "timestamp": 1715988160, "done": true}}, "currentId": "8062f01d-2ce9-44cd-840c-0e9cfe8df1ef"}, "tags": [], "timestamp": 1715988158993}


id: d8397fa7-496a-4a7d-8cde-5c569abc99dd
user_id: 05d069f6-4441-41d7-bbee-ac0277857a6c
title: ### Monet-All Logo Design
chat:
  {"id": "", "title": "### Monet-All Logo Design", "models": ["gpt-4o-all"], "options": {}, "messages": [{"id": "3982051e-a3ee-4943-921d-07f63bf73790", "parentId": null, "childrenIds": ["792dc67b-3068-466f-93ea-a0779d22352f"], "role": "user", "content": "\u8bf7\u9605\u8bfbhttps://monet.jerryz.com.cn\uff0c\u4e3aMonet-all\u753b\u4e00\u4e2a\u56fe\u6807", "timestamp": 1715993495}, {"parentId": "3982051e-a3ee-4943-921d-07f63bf73790", "id": "792dc67b-3068-466f-93ea-a0779d22352f", "childrenIds": ["97c64f5b-be80-41a1-b3ce-0dcd9f429ad3"], "role": "assistant", "content": "Monet All \u662f\u4e00\u4e2a\u9002\u7528\u4e8e Android 12 \u53ca\u66f4\u9ad8\u7248\u672c\u7684\u4e3b\u9898\u53d6\u8272\u6574\u5408\u6a21\u5757\u3002\u5b83\u901a\u8fc7\u58c1\u7eb8\u751f\u6210\u7cfb\u7edf\u4e3b\u9898\u8272\uff0c\u5e76\u4f7f\u5e94\u7528\u4e0e\u4e4b\u5339\u914d\u3002\u7528\u6237\u53ef\u4ee5\u901a\u8fc7 Magisk \u548c KernelSU \u8fdb\u884c\u5b89\u88c5\uff0c\u5e76\u4f7f\u7528\u97f3\u91cf\u952e\u9009\u62e9\u662f\u5426\u5b89\u88c5\u76f8\u5e94\u7684\u53d6\u8272\u6a21\u5757\u3002\u8be5\u9879\u76ee\u662f\u5f00\u6e90\u7684\uff0c\u9075\u5faa MIT \u8bb8\u53ef\u8bc1\u3002\n\nBased on this, I suggest an icon that incorporates elements of Android, customization, and color. The design should be modern and simple, reflecting the \"Material You\" aesthetic.\n\nLet's proceed to create a detailed prompt for the icon.", "model": "gpt-4o-all", "timestamp": 1715993496, "done": true, "files": [{"type": "image", "url": "/cache/image/generations/e94d0205-e8f4-4a18-882b-927e74c38e23.png"}]}, {"id": "97c64f5b-be80-41a1-b3ce-0dcd9f429ad3", "parentId": "792dc67b-3068-466f-93ea-a0779d22352f", "childrenIds": ["e7e24418-bcb4-4029-9e3c-0af5d12e5c3c"], "role": "user", "content": "\u8bf7\u753b\u51fa\u56fe\u6807", "timestamp": 1715993515}, {"parentId": "97c64f5b-be80-41a1-b3ce-0dcd9f429ad3", "id": "e7e24418-bcb4-4029-9e3c-0af5d12e5c3c", "childrenIds": [], "role": "assistant", "content": "![dalle-generations/ab2e21fd-8e75-4efe-a6d2-f05a741be4bb.webp](https://files.oaifree.com/file-klyPKOhCqzPTcP9yXH7YJjUT?se=2024-05-18T00%3A57%3A07Z&sp=r&sv=2023-11-03&sr=b&rscc=max-age%3D31536000%2C%20immutable&rscd=attachment%3B%20filename%3Dab2e21fd-8e75-4efe-a6d2-f05a741be4bb.webp&sig=LeAmyQoutGX%2BQAw0nlk8otQACJbQyFY60nNQLl6vnVs%3D \"Design an icon for Monet All, an Android theme color integration module. The icon should be modern and simple, reflecting the 'Material You' aesthetic. Incorporate elements of Android, customization, and color. Use a color palette that suggests customization and dynamic theming, with smooth gradients and a clean design. The icon should be visually appealing and represent the functionality of generating theme colors from the wallpaper and integrating them across applications.\") \n\u56fe\u6807\u5df2\u7ecf\u7ed8\u5236\u5b8c\u6210\u3002\u8bf7\u67e5\u770b\u4ee5\u4e0a\u751f\u6210\u7684\u56fe\u6807\uff0c\u770b\u770b\u662f\u5426\u7b26\u5408\u60a8\u7684\u9884\u671f\u3002", "model": "gpt-4o-all", "timestamp": 1715993515, "done": true}], "history": {"messages": {"3982051e-a3ee-4943-921d-07f63bf73790": {"id": "3982051e-a3ee-4943-921d-07f63bf73790", "parentId": null, "childrenIds": ["792dc67b-3068-466f-93ea-a0779d22352f"], "role": "user", "content": "\u8bf7\u9605\u8bfbhttps://monet.jerryz.com.cn\uff0c\u4e3aMonet-all\u753b\u4e00\u4e2a\u56fe\u6807", "timestamp": 1715993495}, "792dc67b-3068-466f-93ea-a0779d22352f": {"parentId": "3982051e-a3ee-4943-921d-07f63bf73790", "id": "792dc67b-3068-466f-93ea-a0779d22352f", "childrenIds": ["97c64f5b-be80-41a1-b3ce-0dcd9f429ad3"], "role": "assistant", "content": "Monet All \u662f\u4e00\u4e2a\u9002\u7528\u4e8e Android 12 \u53ca\u66f4\u9ad8\u7248\u672c\u7684\u4e3b\u9898\u53d6\u8272\u6574\u5408\u6a21\u5757\u3002\u5b83\u901a\u8fc7\u58c1\u7eb8\u751f\u6210\u7cfb\u7edf\u4e3b\u9898\u8272\uff0c\u5e76\u4f7f\u5e94\u7528\u4e0e\u4e4b\u5339\u914d\u3002\u7528\u6237\u53ef\u4ee5\u901a\u8fc7 Magisk \u548c KernelSU \u8fdb\u884c\u5b89\u88c5\uff0c\u5e76\u4f7f\u7528\u97f3\u91cf\u952e\u9009\u62e9\u662f\u5426\u5b89\u88c5\u76f8\u5e94\u7684\u53d6\u8272\u6a21\u5757\u3002\u8be5\u9879\u76ee\u662f\u5f00\u6e90\u7684\uff0c\u9075\u5faa MIT \u8bb8\u53ef\u8bc1\u3002\n\nBased on this, I suggest an icon that incorporates elements of Android, customization, and color. The design should be modern and simple, reflecting the \"Material You\" aesthetic.\n\nLet's proceed to create a detailed prompt for the icon.", "model": "gpt-4o-all", "timestamp": 1715993496, "done": true, "files": [{"type": "image", "url": "/cache/image/generations/e94d0205-e8f4-4a18-882b-927e74c38e23.png"}]}, "97c64f5b-be80-41a1-b3ce-0dcd9f429ad3": {"id": "97c64f5b-be80-41a1-b3ce-0dcd9f429ad3", "parentId": "792dc67b-3068-466f-93ea-a0779d22352f", "childrenIds": ["e7e24418-bcb4-4029-9e3c-0af5d12e5c3c"], "role": "user", "content": "\u8bf7\u753b\u51fa\u56fe\u6807", "timestamp": 1715993515}, "e7e24418-bcb4-4029-9e3c-0af5d12e5c3c": {"parentId": "97c64f5b-be80-41a1-b3ce-0dcd9f429ad3", "id": "e7e24418-bcb4-4029-9e3c-0af5d12e5c3c", "childrenIds": [], "role": "assistant", "content": "![dalle-generations/ab2e21fd-8e75-4efe-a6d2-f05a741be4bb.webp](https://files.oaifree.com/file-klyPKOhCqzPTcP9yXH7YJjUT?se=2024-05-18T00%3A57%3A07Z&sp=r&sv=2023-11-03&sr=b&rscc=max-age%3D31536000%2C%20immutable&rscd=attachment%3B%20filename%3Dab2e21fd-8e75-4efe-a6d2-f05a741be4bb.webp&sig=LeAmyQoutGX%2BQAw0nlk8otQACJbQyFY60nNQLl6vnVs%3D \"Design an icon for Monet All, an Android theme color integration module. The icon should be modern and simple, reflecting the 'Material You' aesthetic. Incorporate elements of Android, customization, and color. Use a color palette that suggests customization and dynamic theming, with smooth gradients and a clean design. The icon should be visually appealing and represent the functionality of generating theme colors from the wallpaper and integrating them across applications.\") \n\u56fe\u6807\u5df2\u7ecf\u7ed8\u5236\u5b8c\u6210\u3002\u8bf7\u67e5\u770b\u4ee5\u4e0a\u751f\u6210\u7684\u56fe\u6807\uff0c\u770b\u770b\u662f\u5426\u7b26\u5408\u60a8\u7684\u9884\u671f\u3002", "model": "gpt-4o-all", "timestamp": 1715993515, "done": true}}, "currentId": "e7e24418-bcb4-4029-9e3c-0af5d12e5c3c"}, "tags": [], "timestamp": 1715993495163}


id: 47e8088c-7532-4c56-85fa-e656135b108e
user_id: 05d069f6-4441-41d7-bbee-ac0277857a6c
title:
  ### DNA琼脂糖凝胶电泳操作

  琼脂糖凝胶电泳是实验室中用于分析DNA片段的一种常见技术。下面是详细的操作步骤：

  1. **准备琼脂糖凝胶**：
     - 将适量的琼脂糖粉末加入缓冲液中（例如1%的琼脂糖凝胶）。
     - 加热至完全溶解，冷却至约60°C后，倒入预先准备好的凝胶模具中。
     - 在凝胶凝固前加入梳子形成样品槽。

  2. **准备样品**：
     - 将DNA样品与加载缓冲液混合（通常包括甘油以增加密度和染料以追踪电泳过程）。
     - 短暂加热样品（通常是65°C下10分钟）以消除二级结构。

  3. **进行电泳**：
     - 将凝胶放入电泳槽中，并加入足够的电泳缓冲液以覆盖凝胶。
     - 载入样品到凝胶的样品槽中。
     - 设置适当的电压（一般为5-8 V/cm），进行电泳分离。

  4. **染色和结果观察**：
     - 电泳结束后，将凝胶浸入含有DNA染料的溶液中（如乙酰胺基苯并呋喃或溴化乙锭），通常染色1-30分钟。
     - 使用紫外光照射凝胶，观察和拍摄DNA条带。

  5. **数据分析**：
     - 根据DNA条带的迁移距离与已知DNA标记比较，估算样品中DNA片段的大小。

  通过上述步骤，可以有效地检测和分析实验样品中的DNA片段。

chat:
  {"id": "", "title": "### DNA\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u64cd\u4f5c\n\n\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u662f\u5b9e\u9a8c\u5ba4\u4e2d\u7528\u4e8e\u5206\u6790DNA\u7247\u6bb5\u7684\u4e00\u79cd\u5e38\u89c1\u6280\u672f\u3002\u4e0b\u9762\u662f\u8be6\u7ec6\u7684\u64cd\u4f5c\u6b65\u9aa4\uff1a\n\n1. **\u51c6\u5907\u743c\u8102\u7cd6\u51dd\u80f6**\uff1a\n   - \u5c06\u9002\u91cf\u7684\u743c\u8102\u7cd6\u7c89\u672b\u52a0\u5165\u7f13\u51b2\u6db2\u4e2d\uff08\u4f8b\u59821%\u7684\u743c\u8102\u7cd6\u51dd\u80f6\uff09\u3002\n   - \u52a0\u70ed\u81f3\u5b8c\u5168\u6eb6\u89e3\uff0c\u51b7\u5374\u81f3\u7ea660\u00b0C\u540e\uff0c\u5012\u5165\u9884\u5148\u51c6\u5907\u597d\u7684\u51dd\u80f6\u6a21\u5177\u4e2d\u3002\n   - \u5728\u51dd\u80f6\u51dd\u56fa\u524d\u52a0\u5165\u68b3\u5b50\u5f62\u6210\u6837\u54c1\u69fd\u3002\n\n2. **\u51c6\u5907\u6837\u54c1**\uff1a\n   - \u5c06DNA\u6837\u54c1\u4e0e\u52a0\u8f7d\u7f13\u51b2\u6db2\u6df7\u5408\uff08\u901a\u5e38\u5305\u62ec\u7518\u6cb9\u4ee5\u589e\u52a0\u5bc6\u5ea6\u548c\u67d3\u6599\u4ee5\u8ffd\u8e2a\u7535\u6cf3\u8fc7\u7a0b\uff09\u3002\n   - \u77ed\u6682\u52a0\u70ed\u6837\u54c1\uff08\u901a\u5e38\u662f65\u00b0C\u4e0b10\u5206\u949f\uff09\u4ee5\u6d88\u9664\u4e8c\u7ea7\u7ed3\u6784\u3002\n\n3. **\u8fdb\u884c\u7535\u6cf3**\uff1a\n   - \u5c06\u51dd\u80f6\u653e\u5165\u7535\u6cf3\u69fd\u4e2d\uff0c\u5e76\u52a0\u5165\u8db3\u591f\u7684\u7535\u6cf3\u7f13\u51b2\u6db2\u4ee5\u8986\u76d6\u51dd\u80f6\u3002\n   - \u8f7d\u5165\u6837\u54c1\u5230\u51dd\u80f6\u7684\u6837\u54c1\u69fd\u4e2d\u3002\n   - \u8bbe\u7f6e\u9002\u5f53\u7684\u7535\u538b\uff08\u4e00\u822c\u4e3a5-8 V/cm\uff09\uff0c\u8fdb\u884c\u7535\u6cf3\u5206\u79bb\u3002\n\n4. **\u67d3\u8272\u548c\u7ed3\u679c\u89c2\u5bdf**\uff1a\n   - \u7535\u6cf3\u7ed3\u675f\u540e\uff0c\u5c06\u51dd\u80f6\u6d78\u5165\u542b\u6709DNA\u67d3\u6599\u7684\u6eb6\u6db2\u4e2d\uff08\u5982\u4e59\u9170\u80fa\u57fa\u82ef\u5e76\u544b\u5583\u6216\u6eb4\u5316\u4e59\u952d\uff09\uff0c\u901a\u5e38\u67d3\u82721-30\u5206\u949f\u3002\n   - \u4f7f\u7528\u7d2b\u5916\u5149\u7167\u5c04\u51dd\u80f6\uff0c\u89c2\u5bdf\u548c\u62cd\u6444DNA\u6761\u5e26\u3002\n\n5. **\u6570\u636e\u5206\u6790**\uff1a\n   - \u6839\u636eDNA\u6761\u5e26\u7684\u8fc1\u79fb\u8ddd\u79bb\u4e0e\u5df2\u77e5DNA\u6807\u8bb0\u6bd4\u8f83\uff0c\u4f30\u7b97\u6837\u54c1\u4e2dDNA\u7247\u6bb5\u7684\u5927\u5c0f\u3002\n\n\u901a\u8fc7\u4e0a\u8ff0\u6b65\u9aa4\uff0c\u53ef\u4ee5\u6709\u6548\u5730\u68c0\u6d4b\u548c\u5206\u6790\u5b9e\u9a8c\u6837\u54c1\u4e2d\u7684DNA\u7247\u6bb5\u3002", "models": ["gpt-4-all", "gpt-4o-all", "claude-3-opus-20240229"], "options": {}, "messages": [{"id": "a25669df-a668-4daf-aa8e-f31a72396f8c", "parentId": null, "childrenIds": ["178fcf9e-2e63-4908-9c4c-e297aca20a25", "dd76f84f-e67b-49e8-b970-3b00e408d62a", "a513174a-2d5b-40b2-84e5-cc1b297ef4fc"], "role": "user", "content": "\u5b9e\u9a8c\u5ba4\u4e2d\u68c0\u6d4bdna\u7684\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u5e94\u8be5\u600e\u6837\u64cd\u4f5c\uff1f\u641c\u7d22\u540e\u7528\u4e2d\u6587\u56de\u7b54\uff0c\u5c3d\u53ef\u80fd\u8be6\u7ec6\uff0c\u5e2e\u6211\u5f62\u6210Markdown\u683c\u5f0f\u7684\u7b14\u8bb0"}, {"parentId": "a25669df-a668-4daf-aa8e-f31a72396f8c", "id": "dd76f84f-e67b-49e8-b970-3b00e408d62a", "childrenIds": [], "role": "assistant", "content": "### \u5b9e\u9a8c\u5ba4\u4e2d\u68c0\u6d4bDNA\u7684\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u64cd\u4f5c\u6b65\u9aa4\n\n#### \u6750\u6599\u548c\u8bd5\u5242\u51c6\u5907\n- **\u6750\u6599**: \n  - DNA\u6837\u54c1\n  - 1kb DNA\u5206\u5b50\u91cf\u6807\u51c6\uff08Marker\uff09\n- **\u8bd5\u5242**: \n  - \u743c\u8102\u7cd6\u7c89\n  - 0.5\u00d7TBE\u62161\u00d7TAE\u7f13\u51b2\u6db2\n  - \u6eb4\u5316\u4e59\u952d\uff08Ethidium Bromide, EB\uff09\n  - \u4e0a\u6837\u7f13\u51b2\u6db2\uff08loading buffer\uff09\uff1a0.25%\u6eb4\u915a\u5170\uff0c40%\u8517\u7cd6\n- **\u5668\u5177**: \n  - \u7535\u6cf3\u7cfb\u7edf\uff08\u7535\u6cf3\u4eea\u3001\u6c34\u5e73\u7535\u6cf3\u69fd\u3001\u5236\u80f6\u677f\uff09\n  - \u7d2b\u5916\u900f\u5c04\u4eea\n  - \u79fb\u6db2\u5668\u53ca\u5438\u5934\n  - \u9525\u5f62\u74f6\n\n#### \u64cd\u4f5c\u6b65\u9aa4\n\n1. **\u914d\u5236\u743c\u8102\u7cd6\u51dd\u80f6**:\n   - \u6839\u636e\u9700\u8981\u5206\u79bb\u7684DNA\u7247\u6bb5\u5927\u5c0f\uff0c\u79f0\u53d6\u9002\u91cf\u7684\u743c\u8102\u7cd6\u7c89\uff08\u4f8b\u59821g\uff09\u653e\u5165\u9525\u5f62\u74f6\u4e2d\u3002\n   - \u52a0\u5165\u9002\u91cf\u76840.5\u00d7TBE\u62161\u00d7TAE\u7f13\u51b2\u6db2\uff08\u4f8b\u5982100ml\uff09\u3002\n   - \u5c06\u9525\u5f62\u74f6\u653e\u5165\u5fae\u6ce2\u7089\u4e2d\u52a0\u70ed\u81f3\u743c\u8102\u7cd6\u5b8c\u5168\u6eb6\u89e3\uff0c\u6eb6\u6db2\u53d8\u5f97\u900f\u660e\u3002\n   - \u51b7\u5374\u743c\u8102\u7cd6\u6eb6\u6db2\u81f3\u7ea660\u2103\uff0c\u52a0\u5165\u6eb4\u5316\u4e59\u952d\u81f3\u7ec8\u6d53\u5ea60.5\u00b5g/ml\uff08\u907f\u514d\u5149\u7167\uff09\u3002\n   - \u5728\u6c34\u5e73\u7535\u6cf3\u69fd\u5185\u653e\u7f6e\u5236\u80f6\u677f\uff0c\u5bc6\u5c01\u56db\u5468\uff0c\u5e76\u63d2\u5165\u68b3\u5b50\u3002\n   - \u7f13\u6162\u5012\u5165\u743c\u8102\u7cd6\u6eb6\u6db2\uff0c\u907f\u514d\u6c14\u6ce1\u4ea7\u751f\uff0c\u9759\u7f6e30-40\u5206\u949f\u76f4\u81f3\u51dd\u56fa\u3002\n\n2. **\u51c6\u5907\u7535\u6cf3\u69fd**:\n   - \u5c0f\u5fc3\u5730\u62d4\u51fa\u68b3\u5b50\uff0c\u786e\u4fdd\u51dd\u80f6\u5b54\u5b8c\u6574\u3002\n   - \u5c06\u51dd\u56fa\u7684\u51dd\u80f6\u653e\u5165\u7535\u6cf3\u69fd\u4e2d\u3002\n   - \u52a0\u5165\u7535\u6cf3\u7f13\u51b2\u6db2\uff0c\u6db2\u9762\u5e94\u7a0d\u5fae\u8986\u76d6\u51dd\u80f6\u8868\u9762\u3002\n\n3. **\u4e0a\u6837**:\n   - \u5c06DNA\u6837\u54c1\u4e0e\u4e0a\u6837\u7f13\u51b2\u6db2\u6df7\u5408\uff08\u4f8b\u59825\u00b5l DNA\u6837\u54c1\u52a0\u51651\u00b5l loading buffer\uff09\u3002\n   - \u4f7f\u7528\u79fb\u6db2\u5668\u5c06\u6df7\u5408\u6db2\u5c0f\u5fc3\u5730\u52a0\u5165\u51dd\u80f6\u5b54\u4e2d\uff0c\u907f\u514d\u6c14\u6ce1\u4ea7\u751f\u3002\n\n4. **\u8fd0\u884c\u7535\u6cf3**:\n   - \u63a5\u901a\u7535\u6e90\uff0c\u8bbe\u5b9a\u7535\u538b\u4e3a40-50V\uff0c\u8fd0\u884c30-40\u5206\u949f\u3002\n   - \u786e\u4fdd\u7535\u6cf3\u7f13\u51b2\u6db2\u8986\u76d6\u51dd\u80f6\u8868\u9762\uff0c\u4ee5\u9632\u6b62\u51dd\u80f6\u5e72\u88c2\u3002\n\n5. **\u89c2\u5bdf\u548c\u8bb0\u5f55\u7ed3\u679c**:\n   - \u7535\u6cf3\u7ed3\u675f\u540e\uff0c\u5173\u95ed\u7535\u6e90\u3002\n   - \u5c06\u51dd\u80f6\u79fb\u81f3\u7d2b\u5916\u900f\u5c04\u4eea\u4e0a\u8fdb\u884c\u89c2\u5bdf\uff0c\u5e76\u62cd\u7167\u8bb0\u5f55DNA\u6761\u5e26\u4f4d\u7f6e\u548c\u5927\u5c0f\u3002\n   - \u6839\u636eMarker\u5224\u65adDNA\u7247\u6bb5\u7684\u5927\u5c0f\u3002\n\n#### \u6ce8\u610f\u4e8b\u9879\n- **\u7f13\u51b2\u6db2\u9009\u62e9**: \u5bf9\u4e8e\u5c0f\u7247\u6bb5DNA\uff08<1000 bp\uff09\uff0c\u4f7f\u75281\u00d7TBE\u7f13\u51b2\u6db2\u6548\u679c\u66f4\u597d\uff0c\u800c\u5bf9\u4e8e\u5927DNA\u7247\u6bb5\uff08>12 kb\uff09\uff0c\u5efa\u8bae\u4f7f\u75281\u00d7TAE\u7f13\u51b2\u6db2\u301011\u2020source\u3011\u301012\u2020source\u3011\u3002\n- **\u51dd\u80f6\u6d53\u5ea6\u9009\u62e9**: \u743c\u8102\u7cd6\u6d53\u5ea6\u7684\u9009\u62e9\u5e94\u6839\u636e\u5206\u79bb\u7684DNA\u5927\u5c0f\u8303\u56f4\u8c03\u6574\uff0c\u901a\u5e38\u4f7f\u75280.7%-2%\u7684\u743c\u8102\u7cd6\u6d53\u5ea6\u301013\u2020source\u3011\u301014\u2020source\u3011\u3002\n- **\u5b89\u5168\u9632\u62a4**: \u6eb4\u5316\u4e59\u952d\u5177\u6709\u6bd2\u6027\uff0c\u64cd\u4f5c\u65f6\u9700\u4f69\u6234\u624b\u5957\uff0c\u5e76\u5728\u901a\u98ce\u826f\u597d\u7684\u73af\u5883\u4e2d\u8fdb\u884c\uff0c\u5e9f\u5f03\u7269\u5904\u7406\u9700\u7b26\u5408\u5b89\u5168\u89c4\u5b9a\u301012\u2020source\u3011\u3002\n\n\u4ee5\u4e0a\u662f\u5b9e\u9a8c\u5ba4\u4e2d\u8fdb\u884cDNA\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u7684\u8be6\u7ec6\u64cd\u4f5c\u6b65\u9aa4\uff0c\u786e\u4fdd\u6bcf\u4e00\u6b65\u90fd\u4e25\u683c\u6309\u7167\u89c4\u8303\u6267\u884c\uff0c\u4ee5\u4fdd\u8bc1\u5b9e\u9a8c\u7ed3\u679c\u7684\u51c6\u786e\u6027\u548c\u53ef\u91cd\u590d\u6027\u3002", "model": "gpt-4o-all", "timestamp": 1716015232, "done": true}], "history": {"messages": {"72c945f5-d93d-4dc1-a3bd-4ae085fc8c81": {"id": "72c945f5-d93d-4dc1-a3bd-4ae085fc8c81", "parentId": null, "childrenIds": ["2d9c85f8-17b5-42ce-858f-ac17ae4ba84a", "e90a15fc-2b3c-4c08-bdc0-70d81d9fa9f2", "f7d80195-e205-405c-94fe-2978c77517c5"], "role": "user", "content": "\u8bf7\u5e2e\u6211\u5199\u51fa\u4ece\u5927\u80a0\u6746\u83cc\u4e2d\u63d0\u53d6\u63d2\u5165\u7684\u8d28\u7c92\u7684Markdown\u7b14\u8bb0", "timestamp": 1716003393}, "2d9c85f8-17b5-42ce-858f-ac17ae4ba84a": {"parentId": "72c945f5-d93d-4dc1-a3bd-4ae085fc8c81", "id": "2d9c85f8-17b5-42ce-858f-ac17ae4ba84a", "childrenIds": [], "role": "assistant", "content": "\u5f53\u7136\u53ef\u4ee5\uff01\u4e0b\u9762\u662f\u4e00\u4e2a\u5173\u4e8e\u5982\u4f55\u4ece\u5927\u80a0\u6746\u83cc\u4e2d\u63d0\u53d6\u63d2\u5165\u7684\u8d28\u7c92\u7684\u6b65\u9aa4\u7684Markdown\u7b14\u8bb0\uff1a\n\n```markdown\n# \u4ece\u5927\u80a0\u6746\u83cc\u4e2d\u63d0\u53d6\u8d28\u7c92\u7684\u6b65\u9aa4\n\n\u63d0\u53d6\u8d28\u7c92\u662f\u5206\u5b50\u751f\u7269\u5b66\u4e2d\u5e38\u89c1\u7684\u5b9e\u9a8c\u65b9\u6cd5\uff0c\u7528\u4e8e\u83b7\u53d6\u7ec6\u83cc\u4e2d\u7684\u8d28\u7c92DNA\u3002\u4ee5\u4e0b\u662f\u4ece\u5927\u80a0\u6746\u83cc\u4e2d\u63d0\u53d6\u8d28\u7c92\u7684\u57fa\u672c\u6b65\u9aa4\uff1a\n\n## \u6750\u6599\u548c\u4eea\u5668\n\n- \u57f9\u517b\u7684\u5927\u80a0\u6746\u83cc\n- \u79bb\u5fc3\u673a\n- \u4f4e\u6e29\u51b0\u7bb1\u6216\u51b0\u76d2\n- \u5438\u7ba1\u548c\u5438\u5934\n- EP\u7ba1\n- \u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2\uff08\u4f8b\u5982\uff1aQIAGEN \u8d28\u7c92\u5c0f\u63d0\u8bd5\u5242\u76d2\uff09\n\n## \u6b65\u9aa4\n\n### 1. \u7ec6\u83cc\u57f9\u517b\n\n- \u5728\u542b\u6709\u9002\u5f53\u6297\u751f\u7d20\u7684LB\u57f9\u517b\u57fa\u4e2d\u63a5\u79cd\u5927\u80a0\u6746\u83cc\uff0c37\u00b0C\u6447\u5e8a\u57f9\u517b12-16\u5c0f\u65f6\u3002\n\n### 2. \u6536\u96c6\u7ec6\u83cc\u7ec6\u80de\n\n- \u5c06\u57f9\u517b\u6db2\u8f6c\u79fb\u5230\u79bb\u5fc3\u7ba1\u4e2d\u3002\n- 12000 g\u79bb\u5fc32\u5206\u949f\uff0c\u5f03\u4e0a\u6e05\uff0c\u4fdd\u7559\u83cc\u4f53\u6c89\u6dc0\u3002\n\n### 3. \u88c2\u89e3\u7ec6\u80de\n\n- \u6dfb\u52a0\u88c2\u89e3\u7f13\u51b2\u6db2\uff0c\u5145\u5206\u60ac\u6d6e\u7ec6\u80de\u6c89\u6dc0\u3002\n- \u52a0\u5165\u88c2\u89e3\u9176\uff0c\u8f7b\u8f7b\u632f\u8361\u6df7\u5408\uff0c\u653e\u7f6e\u6570\u5206\u949f\uff0c\u8ba9\u7ec6\u80de\u5145\u5206\u88c2\u89e3\u3002\n\n### 4. \u4e2d\u548c\u88c2\u89e3\n\n- \u52a0\u5165\u4e2d\u548c\u7f13\u51b2\u6db2\uff0c\u5145\u5206\u6df7\u5408\u3002\n- \u79bb\u5fc3\u53bb\u9664\u88c2\u89e3\u540e\u7684\u7ec6\u80de\u788e\u7247\uff0c\u5c06\u542b\u6709\u8d28\u7c92\u7684\u4e0a\u6e05\u8f6c\u79fb\u5230\u65b0\u7684EP\u7ba1\u4e2d\u3002\n\n### 5. \u8d28\u7c92DNA\u7684\u7eaf\u5316\n\n- \u6839\u636e\u8bd5\u5242\u76d2\u8bf4\u660e\u4e66\u64cd\u4f5c\uff0c\u901a\u5e38\u5305\u62ec\u7ed3\u5408\u3001\u6d17\u6da4\u548c\u6d17\u8131\u6b65\u9aa4\u3002\n- \u5c06\u6d17\u8131\u7684\u8d28\u7c92DNA\u50a8\u5b58\u5728-20\u00b0C\u3002\n\n## \u6ce8\u610f\u4e8b\u9879\n\n- \u786e\u4fdd\u5728\u6574\u4e2a\u8fc7\u7a0b\u4e2d\u4f7f\u7528\u65e0\u83cc\u6280\u672f\uff0c\u4ee5\u907f\u514dDNA\u6c61\u67d3\u3002\n- \u5404\u6b65\u9aa4\u7684\u7f13\u51b2\u6db2\u7ec4\u6210\u548c\u5177\u4f53\u6761\u4ef6\u53ef\u80fd\u56e0\u8bd5\u5242\u76d2\u7684\u4e0d\u540c\u800c\u6709\u6240\u4e0d\u540c\uff0c\u8bf7\u8be6\u7ec6\u9605\u8bfb\u4ea7\u54c1\u624b\u518c\u3002\n\n## \u5e94\u7528\n\n- \u63d0\u53d6\u7684\u8d28\u7c92\u53ef\u4ee5\u7528\u4e8e\u514b\u9686\u3001\u5e8f\u5217\u5206\u6790\u3001\u57fa\u56e0\u8868\u8fbe\u7b49\u7814\u7a76\u3002\n\n```\n\n\u8fd9\u4efd\u7b14\u8bb0\u6982\u8ff0\u4e86\u4ece\u5927\u80a0\u6746\u83cc\u4e2d\u63d0\u53d6\u8d28\u7c92\u7684\u57fa\u672c\u6b65\u9aa4\u548c\u6ce8\u610f\u4e8b\u9879\u3002\u5e0c\u671b\u8fd9\u5bf9\u4f60\u7684\u5b9e\u9a8c\u6709\u5e2e\u52a9\uff01", "model": "gpt-4-all", "timestamp": 1716003396, "done": true}, "e90a15fc-2b3c-4c08-bdc0-70d81d9fa9f2": {"parentId": "72c945f5-d93d-4dc1-a3bd-4ae085fc8c81", "id": "e90a15fc-2b3c-4c08-bdc0-70d81d9fa9f2", "childrenIds": [], "role": "assistant", "content": "### \u5927\u80a0\u6746\u83cc\u4e2d\u63d0\u53d6\u63d2\u5165\u8d28\u7c92\u7684\u6b65\u9aa4\n\n#### \u5b9e\u9a8c\u6750\u6599\n- LB\u57f9\u517b\u57fa\n- \u6297\u751f\u7d20\uff08\u6839\u636e\u8d28\u7c92\u4e0a\u7684\u6297\u6027\u57fa\u56e0\u9009\u62e9\u9002\u5f53\u7684\u6297\u751f\u7d20\uff09\n- \u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2\uff08\u5982Qiagen\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2\uff09\n- \u8d85\u7eaf\u6c34\n- \u79bb\u5fc3\u7ba1\uff081.5 mL\u62162 mL\uff09\n- \u5438\u5934\u53ca\u79fb\u6db2\u5668\n- \u79bb\u5fc3\u673a\n- \u7ec6\u83cc\u57f9\u517b\u6447\u5e8a\n\n#### \u5b9e\u9a8c\u6b65\u9aa4\n\n1. **\u7ec6\u83cc\u57f9\u517b**\n   - \u9009\u62e9\u542b\u6709\u63d2\u5165\u8d28\u7c92\u7684\u5927\u80a0\u6746\u83cc\u83cc\u682a\uff0c\u63a5\u79cd\u4e8e\u542b\u6709\u76f8\u5e94\u6297\u751f\u7d20\u7684LB\u57f9\u517b\u57fa\u4e2d\u3002\n   - \u572837\u00b0C\u6447\u5e8a\u4e2d\u57f9\u517b\u8fc7\u591c\uff0812-16\u5c0f\u65f6\uff09\u3002\n\n2. **\u7ec6\u80de\u6536\u96c6**\n   - \u5c06\u8fc7\u591c\u57f9\u517b\u7684\u83cc\u6db2\u5012\u51651.5 mL\u62162 mL\u7684\u79bb\u5fc3\u7ba1\u4e2d\u3002\n   - \u4ee512,000 rpm\u79bb\u5fc32\u5206\u949f\uff0c\u5f03\u4e0a\u6e05\uff0c\u5c3d\u91cf\u53bb\u9664\u6b8b\u7559\u57f9\u517b\u57fa\u3002\n\n3. **\u7ec6\u80de\u88c2\u89e3**\n   - \u6839\u636e\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2\u7684\u8bf4\u660e\uff0c\u52a0\u5165\u9002\u91cf\u88c2\u89e3\u7f13\u51b2\u6db2\uff08\u901a\u5e38\u662f\u6eb6\u83cc\u9176\u6eb6\u6db2\u6216\u78b1\u88c2\u89e3\u6db2\uff09\u3002\n   - \u8f7b\u8f7b\u98a0\u5012\u6df7\u5300\uff0c\u9759\u7f6e5\u5206\u949f\u3002\n\n4. **\u4e2d\u548c**\n   - \u52a0\u5165\u4e2d\u548c\u7f13\u51b2\u6db2\uff0c\u8f7b\u8f7b\u98a0\u5012\u6df7\u5300\uff0c\u76f4\u81f3\u6eb6\u6db2\u53d8\u4e3a\u5747\u5300\u7684\u767d\u8272\u6df7\u6d4a\u7269\u3002\n\n5. **\u79bb\u5fc3**\n   - \u4ee512,000 rpm\u79bb\u5fc310\u5206\u949f\uff0c\u6536\u96c6\u4e0a\u6e05\u6db2\uff08\u542b\u6709\u8d28\u7c92DNA\uff09\u3002\n\n6. **DNA\u7ed3\u5408**\n   - \u5c06\u4e0a\u6e05\u6db2\u8f6c\u79fb\u81f3\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2\u63d0\u4f9b\u7684DNA\u7ed3\u5408\u67f1\u4e2d\u3002\n   - \u4ee512,000 rpm\u79bb\u5fc31\u5206\u949f\uff0c\u5f03\u6d41\u51fa\u6db2\u3002\n\n7. **\u6e05\u6d17**\n   - \u5411DNA\u7ed3\u5408\u67f1\u4e2d\u52a0\u5165\u6d17\u6da4\u7f13\u51b2\u6db2\uff08\u542b\u4e59\u9187\uff09\uff0c\u4ee512,000 rpm\u79bb\u5fc31\u5206\u949f\uff0c\u5f03\u6d41\u51fa\u6db2\u3002\n   - \u91cd\u590d\u6d17\u6da4\u6b65\u9aa4\u4e00\u6b21\u3002\n\n8. **\u5e72\u71e5**\n   - \u518d\u6b21\u4ee512,000 rpm\u79bb\u5fc31\u5206\u949f\uff0c\u4ee5\u53bb\u9664\u6b8b\u4f59\u7684\u6d17\u6da4\u7f13\u51b2\u6db2\u3002\n\n9. **\u6d17\u8131**\n   - \u5c06DNA\u7ed3\u5408\u67f1\u7f6e\u4e8e\u65b0\u7684\u79bb\u5fc3\u7ba1\u4e2d\uff0c\u52a0\u5165\u9002\u91cf\u7684\u6d17\u8131\u7f13\u51b2\u6db2\uff08\u5982\u8d85\u7eaf\u6c34\u6216TE\u7f13\u51b2\u6db2\uff09\u3002\n   - \u9759\u7f6e1\u5206\u949f\uff0c\u4ee512,000 rpm\u79bb\u5fc31\u5206\u949f\uff0c\u6536\u96c6\u6d17\u8131\u6db2\uff08\u5373\u8d28\u7c92DNA\u6eb6\u6db2\uff09\u3002\n\n10. **\u8d28\u7c92DNA\u6d53\u5ea6\u53ca\u7eaf\u5ea6\u6d4b\u5b9a**\n    - \u4f7f\u7528NanoDrop\u6216\u5176\u4ed6\u7d2b\u5916\u5206\u5149\u5149\u5ea6\u8ba1\u6d4b\u5b9a\u8d28\u7c92DNA\u7684\u6d53\u5ea6\u53ca\u7eaf\u5ea6\uff08A260/A280\u6bd4\u503c\uff09\u3002\n\n#### \u6ce8\u610f\u4e8b\u9879\n- \u64cd\u4f5c\u8fc7\u7a0b\u4e2d\u5e94\u5c3d\u91cf\u907f\u514dDNA\u964d\u89e3\uff0c\u53ef\u9009\u62e9\u5728\u51b0\u4e0a\u8fdb\u884c\u90e8\u5206\u6b65\u9aa4\u3002\n- \u8d28\u7c92\u63d0\u53d6\u7684\u6574\u4e2a\u8fc7\u7a0b\u5e94\u4fdd\u6301\u65e0\u83cc\uff0c\u4ee5\u9632\u6b62\u6837\u54c1\u6c61\u67d3\u3002\n\n#### \u5e38\u89c1\u95ee\u9898\u53ca\u89e3\u51b3\u65b9\u6cd5\n- **\u8d28\u7c92\u4ea7\u91cf\u4f4e**\uff1a\u53ef\u80fd\u662f\u7ec6\u80de\u88c2\u89e3\u4e0d\u5b8c\u5168\uff0c\u53ef\u5c1d\u8bd5\u5ef6\u957f\u88c2\u89e3\u65f6\u95f4\u6216\u4f7f\u7528\u66f4\u9ad8\u6d53\u5ea6\u7684\u88c2\u89e3\u7f13\u51b2\u6db2\u3002\n- **\u8d28\u7c92\u7eaf\u5ea6\u4f4e**\uff1a\u6d17\u6da4\u6b65\u9aa4\u4e0d\u5145\u5206\uff0c\u53ef\u589e\u52a0\u6d17\u6da4\u7f13\u51b2\u6db2\u7684\u4f53\u79ef\u6216\u6d17\u6da4\u6b21\u6570\u3002\n\n\u901a\u8fc7\u4e0a\u8ff0\u6b65\u9aa4\uff0c\u4f60\u5e94\u8be5\u80fd\u591f\u6210\u529f\u5730\u4ece\u5927\u80a0\u6746\u83cc\u4e2d\u63d0\u53d6\u51fa\u63d2\u5165\u7684\u8d28\u7c92\uff0c\u5e76\u7528\u4e8e\u540e\u7eed\u7684\u5206\u5b50\u751f\u7269\u5b66\u5b9e\u9a8c\u3002", "model": "gpt-4o-all", "timestamp": 1716003396, "done": true}, "f7d80195-e205-405c-94fe-2978c77517c5": {"parentId": "72c945f5-d93d-4dc1-a3bd-4ae085fc8c81", "id": "f7d80195-e205-405c-94fe-2978c77517c5", "childrenIds": [], "role": "assistant", "content": "Here is a Markdown guide for extracting plasmid DNA from E. coli:\n\n#### Materials\n- LB broth and LB agar plates with appropriate antibiotic\n- Sterile pipette tips and serological pipettes\n- Microcentrifuge tubes \n- Miniprep kit (e.g. Qiagen QIAprep Spin Miniprep Kit)\n\n#### Procedure\n1. Grow a 3-5 mL overnight culture of your E. coli containing the plasmid in LB broth with antibiotic. Incubate at 37\u00b0C with shaking.\n\n2. Pellet 1-5 mL of the culture by centrifuging at >8000 rpm for 3 min at room temperature. Discard supernatant.\n\n3. Resuspend pelleted bacterial cells in 250 \u03bcL Buffer P1 (with RNase A added) and transfer to a microcentrifuge tube. No cell clumps should be visible after resuspension.\n\n4. Add 250 \u03bcL Buffer P2 and mix thoroughly by inverting 4-6 times until solution becomes clear. Do not allow lysis reaction to proceed for more than 5 min. \n\n5. Add 350 \u03bcL Buffer N3 and mix immediately by inverting 4-6 times. A white, cloudy precipitate should form.\n\n6. Centrifuge for 10 min at 13,000 rpm in a table-top microcentrifuge.\n\n7. Apply the supernatant from step 6 to a QIAprep 2.0 spin column by pipetting.\n\n8. Centrifuge for 30-60 s and discard the flow-through.\n\n9. Wash the spin column by adding 0.5 mL Buffer PB and centrifuging for 30-60 s. Discard flow-through.\n\n10. Wash column by adding 0.75 mL Buffer PE. Let stand 2-5 min and centrifuge 30-60 s.\n\n11. Discard flow-through and centrifuge at full speed for an additional 1 min to remove residual wash buffer.\n\n12. Place column in a clean 1.5 mL microcentrifuge tube. To elute DNA, add 50 \u03bcL Buffer EB or water to center of column, let stand for 1 min, and centrifuge for 1 min.\n\nThe resulting eluate contains your purified plasmid DNA. Store at -20\u00b0C or use immediately for downstream applications. Quantify yield by UV spectrophotometry if needed.", "model": "claude-3-opus-20240229", "timestamp": 1716003396, "done": true}, "cb623b70-cd3c-43f1-88ad-b01802b5dc8c": {"id": "cb623b70-cd3c-43f1-88ad-b01802b5dc8c", "parentId": null, "childrenIds": ["491ac011-9946-4572-a072-f59126180d4d", "7ef36b08-0ef6-4e9a-8450-5c3c76954564", "9089e629-de0b-47ec-a48d-98686e939a13"], "role": "user", "content": "\u8bf7\u662f\u4e00\u4e2a\u6709\u7528\u7684\u7b14\u8bb0\u52a9\u624b\uff0c\u8bf7\u5e2e\u6211\u5199\u51fa\u4ece\u5927\u80a0\u6746\u83cc\u4e2d\u63d0\u53d6\u63d2\u5165\u7684\u8d28\u7c92\u7684Markdown\u7b14\u8bb0\uff0c\u4f7f\u7528Omega\u63d0\u4f9b\u7684\u8bd5\u5242\u76d2\uff0c\u53ef\u4ee5\u641c\u7d22\u83b7\u5f97\u66f4\u591a\u4fe1\u606f"}, "491ac011-9946-4572-a072-f59126180d4d": {"parentId": "cb623b70-cd3c-43f1-88ad-b01802b5dc8c", "id": "491ac011-9946-4572-a072-f59126180d4d", "childrenIds": [], "role": "assistant", "content": "Here is a simplified markdown note format for extracting plasmid DNA from E. coli using the Omega Bio-tek E.Z.N.A.\u00ae Plasmid DNA Mini Kit I or II. These kits are designed for rapid and efficient isolation of high-quality plasmid DNA using a silica spin column technology under optimized conditions.\n\n### Plasmid DNA Extraction Using Omega E.Z.N.A.\u00ae Plasmid DNA Mini Kit\n\n#### Materials Needed:\n- E.Z.N.A.\u00ae Plasmid DNA Mini Kit I or II\n- 1-5 mL overnight E. coli culture in LB medium\n- Microcentrifuge\n- Optional: Vacuum manifold for vacuum protocol\n\n#### Procedure:\n1. **Preparation**: Grow E. coli in LB medium overnight.\n2. **Lysis**:\n   - Add Solution I (contains RNase A) to the culture.\n   - Add Solution II to lyse the cells.\n   - Neutralize the lysate with Solution III.\n3. **Clearance**:\n   - Centrifuge to clear the lysate.\n   - Optionally, use a vacuum manifold to draw the cleared lysate through the column.\n4. **DNA Binding**:\n   - Transfer the cleared lysate to the HiBind\u00ae DNA Mini Column.\n   - Centrifuge or apply vacuum to bind DNA to the column.\n5. **Washing**:\n   - Wash the column with wash buffer to remove contaminants.\n6. **Elution**:\n   - Elute the purified plasmid DNA with Elution Buffer.\n\n#### Expected Results:\n- Yield: 15-25 \u00b5g high-copy number plasmid DNA from Mini Kit I; 40-70 \u00b5g from Mini Kit II.\n- Time: Less than 30 minutes.\n\n#### Applications:\n- Suitable for cloning, sequencing, PCR, restriction digestion, and other molecular biology applications.\n\nFor a detailed step-by-step protocol, refer to the manufacturer\u2019s guide provided with the kit. These kits ensure a safe procedure without the use of phenol/chloroform, offering a rapid and efficient method to obtain high-quality DNA suitable for a variety of downstream applications\u30107\u2020source\u3011\u30108\u2020source\u3011.", "model": "gpt-4-all", "timestamp": 1716003504, "done": true}, "7ef36b08-0ef6-4e9a-8450-5c3c76954564": {"parentId": "cb623b70-cd3c-43f1-88ad-b01802b5dc8c", "id": "7ef36b08-0ef6-4e9a-8450-5c3c76954564", "childrenIds": [], "role": "assistant", "content": "\u4ee5\u4e0b\u662f\u4f7f\u7528Omega E.Z.N.A.\u00ae Plasmid DNA Mini Kit\u4ece\u5927\u80a0\u6746\u83cc\u4e2d\u63d0\u53d6\u63d2\u5165\u8d28\u7c92\u7684Markdown\u7b14\u8bb0\uff1a\n\n# \u4f7f\u7528Omega E.Z.N.A.\u00ae Plasmid DNA Mini Kit\u63d0\u53d6\u8d28\u7c92\u7684\u6b65\u9aa4\n\n## \u6750\u6599\u548c\u8bbe\u5907\n\n- **\u8bd5\u5242\u76d2**: Omega E.Z.N.A.\u00ae Plasmid DNA Mini Kit\n- **\u57f9\u517b\u57fa**: \u542b\u9002\u5f53\u6297\u751f\u7d20\u7684LB\u57f9\u517b\u57fa\n- **\u79bb\u5fc3\u673a**: \u81f3\u5c1113,000 x g\n- **\u5fae\u91cf\u79bb\u5fc3\u7ba1**: 1.5 mL\u62162 mL\u65e0\u6838\u9178\u9176\u5fae\u91cf\u79bb\u5fc3\u7ba1\n- **\u5176\u4ed6**: 100%\u4e59\u9187\u300170\u00b0C\u6c34\u6d74\u6216\u57f9\u517b\u7bb1\uff08\u53ef\u9009\uff09\n\n## \u8bd5\u5242\u51c6\u5907\n\n1. **RNase A**:\n   - \u5c06RNase A\u52a0\u5165Solution I\u4e2d\uff0c\u5b58\u50a8\u4e8e2-8\u00b0C\uff08\u4ec5\u9002\u7528\u4e8e50\u6b21\u548c200\u6b21\u8bd5\u5242\u76d2\uff09\u3002\n2. **DNA Wash Buffer**:\n   - \u6839\u636e\u74f6\u5b50\u8bf4\u660e\u7528100%\u4e59\u9187\u7a00\u91ca\uff0c\u5b58\u50a8\u4e8e\u5ba4\u6e29\u3002\n3. **Solution II\u548cSolution III**:\n   - \u4f7f\u7528\u524d\u68c0\u67e5\u662f\u5426\u6709\u6c89\u6dc0\uff0c\u5982\u6709\uff0c37\u00b0C\u52a0\u70ed\u6eb6\u89e3\u3002\n\n## \u57f9\u517b\u548c\u88c2\u89e3\u6b65\u9aa4\n\n1. **\u57f9\u517b\u7ec6\u83cc**:\n   - \u4ece\u65b0\u5212\u7ebf\u7684\u9009\u62e9\u6027\u5e73\u677f\u4e2d\u6311\u53d6\u5355\u4e2a\u83cc\u843d\uff0c\u63a5\u79cd1-5 mL\u542b\u9002\u5f53\u6297\u751f\u7d20\u7684LB\u57f9\u517b\u57fa\u4e2d\u300237\u00b0C\u3001300 rpm\u632f\u8361\u57f9\u517b12-16\u5c0f\u65f6\u3002\n2. **\u6536\u96c6\u7ec6\u83cc**:\n   - \u4ee510,000 x g\u79bb\u5fc31\u5206\u949f\uff0c\u5f03\u53bb\u57f9\u517b\u57fa\u3002\n3. **\u88c2\u89e3\u7ec6\u80de**:\n   - \u52a0\u5165250 \u03bcL Solution I/RNase A\uff0c\u5145\u5206\u6da1\u65cb\u6df7\u5300\u3002\n\n## \u8d28\u7c92\u63d0\u53d6\u6b65\u9aa4\n\n1. **\u52a0Solution II**:\n   - \u52a0\u5165250 \u03bcL Solution II\uff0c\u8f7b\u67d4\u4e0a\u4e0b\u98a0\u5012\u6df7\u530010\u6b21\uff0c\u5ba4\u6e29\u653e\u7f6e2-3\u5206\u949f\u3002\n2. **\u52a0Solution III**:\n   - \u52a0\u5165350 \u03bcL Solution III\uff0c\u7acb\u5373\u4e0a\u4e0b\u98a0\u5012\u6df7\u530010\u6b21\uff0c4\u00b0C\u79bb\u5fc310\u5206\u949f\u3002\n3. **\u7ed1\u5b9aDNA**:\n   - \u5c06\u4e0a\u6e05\u6db2\u8f6c\u79fb\u81f3HiBind\u00ae DNA Mini Column\u4e2d\uff0c13,000 x g\u79bb\u5fc31\u5206\u949f\uff0c\u5f03\u53bb\u6d41\u51fa\u6db2\u3002\n4. **\u6e05\u6d17DNA**:\n   - \u52a0\u5165500 \u03bcL HBC Buffer\uff0c13,000 x g\u79bb\u5fc31\u5206\u949f\uff0c\u5f03\u53bb\u6d41\u51fa\u6db2\u3002\n   - \u52a0\u5165700 \u03bcL DNA Wash Buffer\uff0c13,000 x g\u79bb\u5fc31\u5206\u949f\uff0c\u91cd\u590d\u4e00\u6b21\u3002\n5. **\u6d17\u8131DNA**:\n   - \u5c06\u67f1\u5b50\u8f6c\u79fb\u5230\u65b0\u7684\u79bb\u5fc3\u7ba1\u4e2d\uff0c\u52a0\u516550-100 \u03bcL Elution Buffer\uff0c\u5ba4\u6e29\u653e\u7f6e1\u5206\u949f\uff0c13,000 x g\u79bb\u5fc31\u5206\u949f\u3002\n\n## \u5b58\u50a8\u548c\u7a33\u5b9a\u6027\n\n- Solution I\uff08\u52a0RNase A\u540e\uff09\u5b58\u50a8\u4e8e2-8\u00b0C\u3002\n- \u5176\u4ed6\u6eb6\u6db2\u5b58\u50a8\u4e8e\u5ba4\u6e29\uff0c\u4f7f\u7528\u540e\u786e\u4fddSolution II\u76d6\u7d27\u3002\n\n\u4ee5\u4e0a\u6b65\u9aa4\u53ef\u4ee5\u572830\u5206\u949f\u5185\u5b8c\u6210\uff0c\u9ad8\u6548\u63d0\u53d6\u8d28\u7c92DNA\uff0c\u9002\u7528\u4e8e\u514b\u9686\u3001\u6d4b\u5e8f\u3001PCR\u7b49\u4e0b\u6e38\u5e94\u7528\u30107\u2020source\u3011\u30108\u2020source\u3011\u3002", "model": "gpt-4o-all", "timestamp": 1716003504, "done": true, "files": [{"type": "image", "url": "/cache/image/generations/2ad6bf37-6ba6-4e7f-b5f6-9d202eda9919.png"}]}, "9089e629-de0b-47ec-a48d-98686e939a13": {"parentId": "cb623b70-cd3c-43f1-88ad-b01802b5dc8c", "id": "9089e629-de0b-47ec-a48d-98686e939a13", "childrenIds": [], "role": "assistant", "content": "# \u4ece\u5927\u80a0\u6746\u83cc\u4e2d\u63d0\u53d6\u8d28\u7c92\u7684\u5b9e\u9a8c\u6b65\u9aa4\n\n## \u6750\u6599\u51c6\u5907\n- \u542b\u6709\u76ee\u7684\u8d28\u7c92\u7684\u5927\u80a0\u6746\u83cc\u83cc\u6db2\n- Omega\u63d0\u4f9b\u7684\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2\uff0c\u5305\u62ec:\n  - \u7ec6\u80de\u88c2\u89e3\u6db2 (Solution I) \n  - \u78b1\u53d8\u6027\u6db2 (Solution II)\n  - \u4e2d\u548c\u6db2 (Solution III)\n  - \u6d17\u6da4\u7f13\u51b2\u6db2 (Wash Buffer) \n  - \u6d17\u8131\u7f13\u51b2\u6db2 (Elution Buffer)\n  - \u5438\u9644\u67f1 (Adsorption Column)\n  - \u79bb\u5fc3\u7ba1\u7b49\u8017\u6750\n- \u65e0\u83cc\u6c34\u3001\u65e0\u83cc\u79bb\u5fc3\u7ba1\u3001\u79fb\u6db2\u5668\u7b49\u5e38\u89c4\u5b9e\u9a8c\u5668\u6750\n\n## \u5b9e\u9a8c\u6b65\u9aa4\n\n1. \u53d62-5ml\u8fc7\u591c\u57f9\u517b\u7684\u542b\u8d28\u7c92\u5927\u80a0\u6746\u83cc\u83cc\u6db2\uff0c12000rpm\u79bb\u5fc31min\uff0c\u5f03\u4e0a\u6e05\u3002\n\n2. \u52a0\u5165250\u03bcl Solution I\uff0c\u8f7b\u8f7b\u6df7\u5300\u81f3\u83cc\u4f53\u5b8c\u5168\u60ac\u6d6e\u3002\n\n3. \u52a0\u5165250\u03bcl Solution II\uff0c\u8f7b\u8f7b\u98a0\u5012\u6df7\u53004-6\u6b21\uff0c\u5ba4\u6e29\u653e\u7f6e2-3min\uff0c\u4f7f\u7ec6\u80de\u5145\u5206\u88c2\u89e3\u3002\u6b64\u65f6\u6eb6\u6db2\u7531\u6d51\u6d4a\u53d8\u5f97\u6e05\u4eae\u7c98\u7a20\u3002\n\n4. \u52a0\u5165350\u03bcl\u9884\u51b7\u7684Solution III\uff0c\u7acb\u5373\u8f7b\u8f7b\u98a0\u5012\u6df7\u5300\uff0c\u767d\u8272\u6c89\u6dc0\u751f\u6210\u3002\u51b0\u6d74\u653e\u7f6e5min\u3002\n\n5. 12000rpm\u79bb\u5fc310min\uff0c\u5c0f\u5fc3\u5438\u53d6\u4e0a\u6e05\u6db2\u5230\u5438\u9644\u67f1\u4e2d\u3002\n\n6. 12000rpm\u79bb\u5fc330-60s\uff0c\u5f03\u5e9f\u6db2\u3002\u52a0\u5165500\u03bcl Wash Buffer\uff0c12000rpm\u79bb\u5fc330-60s\uff0c\u91cd\u590d\u6d17\u6da4\u4e00\u6b21\u3002\n\n7. 12000rpm\u79bb\u5fc32min\uff0c\u5c3d\u53ef\u80fd\u53bb\u9664\u6b8b\u7559\u7684Wash Buffer\u3002\n\n8. \u5c06\u5438\u9644\u67f1\u8f6c\u79fb\u5230\u65b0\u7684\u79bb\u5fc3\u7ba1\u4e2d\uff0c\u52a0\u516550-100\u03bcl Elution Buffer\u6216\u65e0\u83cc\u6c34\uff0c\u5ba4\u6e29\u653e\u7f6e2min\u3002\n\n9. 12000rpm\u79bb\u5fc32min\uff0c\u6536\u96c6\u6d17\u8131\u7684\u8d28\u7c92DNA\u6eb6\u6db2\u3002-20\u00b0C\u4fdd\u5b58\u5907\u7528\u3002\n\n## \u6ce8\u610f\u4e8b\u9879\n1. \u5b9e\u9a8c\u8fc7\u7a0b\u907f\u514dDNA\u9176\u6c61\u67d3\uff0c\u4f7f\u7528\u65e0\u83cc\u64cd\u4f5c\u3002\n2. Solution II\u52a0\u5165\u540e\u8981\u8fc5\u901f\u6df7\u5300\uff0c\u907f\u514d\u5c40\u90e8\u78b1\u53d8\u6027\u65f6\u95f4\u8fc7\u957f\u3002\n3. \u6d17\u8131\u65f6Elution Buffer\u9884\u70ed\u81f365\u00b0C\u53ef\u63d0\u9ad8\u6d17\u8131\u6548\u7387\u3002\n4. \u8d28\u7c92\u6d17\u8131\u6db2\u5e94\u907f\u5149\u4fdd\u5b58\uff0c-20\u00b0C\u53ef\u957f\u671f\u4fdd\u5b58\u3002\n\n\u4ee5\u4e0a\u662f\u4f7f\u7528Omega\u8bd5\u5242\u76d2\u4ece\u5927\u80a0\u6746\u83cc\u4e2d\u63d0\u53d6\u8d28\u7c92\u7684\u57fa\u672c\u6d41\u7a0b\uff0c\u4e0d\u540c\u5382\u5bb6\u7684\u8bd5\u5242\u76d2\u64cd\u4f5c\u53ef\u80fd\u7565\u6709\u5dee\u5f02\uff0c\u5177\u4f53\u4ee5\u8bf4\u660e\u4e66\u4e3a\u51c6\u3002\u63d0\u53d6\u7684\u8d28\u7c92\u53ef\u7528\u4e8e\u540e\u7eed\u7684\u9176\u5207\u3001PCR\u3001\u6d4b\u5e8f\u7b49\u5206\u5b50\u751f\u7269\u5b66\u5b9e\u9a8c\u3002", "model": "claude-3-opus-20240229", "timestamp": 1716003504, "done": true}, "680ced3e-1e3b-4c68-af25-8fd55dc74435": {"id": "680ced3e-1e3b-4c68-af25-8fd55dc74435", "parentId": null, "childrenIds": ["b6c7dfae-c8de-48ab-a013-84aee4cdd97a", "9905e853-c94e-4adc-a9b8-1b7bca565891", "968cfd61-75a4-490a-8e51-7fe9d07a2668"], "role": "user", "content": "\u5b9e\u9a8c\u5ba4\u51dd\u80f6\u7535\u6cf3\u4e2d\u7528\u5230\u7684\u51dd\u80f6\u4e00\u822c\u5e94\u8be5\u600e\u6837\u5236\u5907\uff1f\u641c\u7d22\u540e\u7528\u4e2d\u6587\u56de\u7b54\uff0c\u5c3d\u53ef\u80fd\u8be6\u7ec6"}, "b6c7dfae-c8de-48ab-a013-84aee4cdd97a": {"parentId": "680ced3e-1e3b-4c68-af25-8fd55dc74435", "id": "b6c7dfae-c8de-48ab-a013-84aee4cdd97a", "childrenIds": [], "role": "assistant", "content": "\u5728\u5b9e\u9a8c\u5ba4\u4e2d\u5236\u5907\u7528\u4e8e\u51dd\u80f6\u7535\u6cf3\u7684\u51dd\u80f6\uff0c\u901a\u5e38\u9700\u8981\u6839\u636e\u8981\u5206\u79bb\u7684\u6837\u672c\u7c7b\u578b\u9009\u62e9\u5408\u9002\u7684\u51dd\u80f6\u7c7b\u578b\u3002\u4e3b\u8981\u6709\u4e24\u79cd\u5e38\u7528\u7684\u51dd\u80f6\uff1a\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u548c\u743c\u8102\u7cd6\u51dd\u80f6\u3002\n\n1. **\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u5236\u5907**\uff08\u5e38\u7528\u4e8e\u86cb\u767d\u8d28\u5206\u79bb\uff09\uff1a\n   - \u51c6\u5907\u6d53\u7f29\u80f6\u548c\u5206\u79bb\u80f6\u7684\u6df7\u5408\u6db2\uff0c\u6d89\u53ca\u5230\u7cbe\u786e\u7684\u6d4b\u91cf\u548c\u6df7\u5408\u4e19\u70ef\u9170\u80fa\u3001\u53cc\u4e19\u70ef\u9170\u80fa\u3001Tris\u7f13\u51b2\u6db2\u3001SDS\u548c\u5176\u4ed6\u5316\u5b66\u54c1\u3002\n   - \u6e05\u6d17\u548c\u88c5\u914d\u73bb\u7483\u677f\uff0c\u5c06\u6df7\u5408\u6db2\u5012\u5165\u6a21\u5177\u4e2d\uff0c\u5e76\u4f7f\u5176\u5728\u5ba4\u6e29\u4e0b\u805a\u5408\u5f62\u6210\u51dd\u80f6\u3002\n   - \u4f7f\u7528\u7535\u6cf3\u524d\u7684\u51c6\u5907\u6db2\uff0c\u5982\u4e0a\u6837\u7f13\u51b2\u6db2\u548c\u7535\u6781\u7f13\u51b2\u6db2\uff0c\u786e\u4fdd\u6837\u54c1\u548c\u7535\u6cf3\u8fc7\u7a0b\u7684\u7a33\u5b9a\u6027\u30108\u2020source\u3011\u301010\u2020source\u3011\u3002\n\n2. **\u743c\u8102\u7cd6\u51dd\u80f6\u5236\u5907**\uff08\u5e38\u7528\u4e8e\u6838\u9178\u5206\u79bb\uff09\uff1a\n   - \u9009\u7528\u9002\u5f53\u6d53\u5ea6\u7684\u743c\u8102\u7cd6\u7c89\uff0c\u6839\u636e\u6240\u9700\u7684\u5206\u5b50\u7b5b\u5206\u6548\u679c\u8c03\u6574\u3002\n   - \u743c\u8102\u7cd6\u7c89\u52a0\u5165\u7f13\u51b2\u6db2\u4e2d\u52a0\u70ed\u81f3\u5b8c\u5168\u6eb6\u89e3\uff0c\u7136\u540e\u51b7\u5374\u81f3\u9002\u5f53\u6e29\u5ea6\u4f7f\u5176\u51dd\u56fa\u6210\u51dd\u80f6\u3002\n   - \u51dd\u80f6\u7684\u5b54\u5f84\u53ef\u4ee5\u901a\u8fc7\u8c03\u6574\u743c\u8102\u7cd6\u7684\u6d53\u5ea6\u6765\u63a7\u5236\uff0c\u9002\u7528\u4e8e\u4e0d\u540c\u5927\u5c0f\u7684DNA\u6216RNA\u7247\u6bb5\u7684\u5206\u79bb\u30109\u2020source\u3011\u3002\n\n\u5728\u5b9e\u9a8c\u64cd\u4f5c\u8fc7\u7a0b\u4e2d\uff0c\u786e\u4fdd\u4f7f\u7528\u7cbe\u786e\u7684\u6d53\u5ea6\u548c\u4f53\u79ef\u662f\u5173\u952e\uff0c\u8fd9\u5f71\u54cd\u5230\u51dd\u80f6\u7684\u5b54\u9699\u5927\u5c0f\u53ca\u5176\u5bf9\u6837\u54c1\u5206\u79bb\u7684\u6548\u679c\u3002\u5236\u5907\u8fc7\u7a0b\u4e2d\u7684\u6e29\u5ea6\u63a7\u5236\u3001pH\u503c\u8c03\u6574\u548c\u5316\u5b66\u54c1\u7684\u7eaf\u5ea6\u4e5f\u975e\u5e38\u91cd\u8981\uff0c\u4ee5\u786e\u4fdd\u7535\u6cf3\u7ed3\u679c\u7684\u91cd\u590d\u6027\u548c\u51c6\u786e\u6027\u3002\u5728\u7535\u6cf3\u8fc7\u7a0b\u4e2d\uff0c\u8fd8\u9700\u8981\u6ce8\u610f\u7535\u538b\u548c\u7535\u6d41\u7684\u63a7\u5236\uff0c\u4ee5\u9632\u51dd\u80f6\u8fc7\u70ed\u6216\u672a\u80fd\u6709\u6548\u5206\u79bb\u6837\u54c1\u301011\u2020source\u3011\u3002", "model": "gpt-4-all", "timestamp": 1716008460, "done": true}, "9905e853-c94e-4adc-a9b8-1b7bca565891": {"parentId": "680ced3e-1e3b-4c68-af25-8fd55dc74435", "id": "9905e853-c94e-4adc-a9b8-1b7bca565891", "childrenIds": [], "role": "assistant", "content": "\u5b9e\u9a8c\u5ba4\u4e2d\u5e38\u7528\u7684\u51dd\u80f6\u7535\u6cf3\u51dd\u80f6\u6709\u743c\u8102\u7cd6\u51dd\u80f6\u548c\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u4e24\u79cd\uff0c\u5236\u5907\u65b9\u6cd5\u5404\u6709\u4e0d\u540c\u3002\u4e0b\u9762\u662f\u8fd9\u4e24\u79cd\u51dd\u80f6\u7684\u8be6\u7ec6\u5236\u5907\u65b9\u6cd5\uff1a\n\n### \u743c\u8102\u7cd6\u51dd\u80f6\n1. **\u914d\u5236\u7f13\u51b2\u6db2**\uff1a\u5e38\u7528\u7684\u7f13\u51b2\u6db2\u662fTris-\u4e59\u9178\u76d0-EDTA\uff08TAE\uff09\u6216Tris-\u787c\u9178\u76d0-EDTA\uff08TBE\uff09\u3002\n2. **\u6eb6\u89e3\u743c\u8102\u7cd6**\uff1a\u6839\u636e\u6240\u9700\u7684\u6d53\u5ea6\uff08\u901a\u5e38\u57280.5%\u52302%\u4e4b\u95f4\uff09\uff0c\u79f0\u53d6\u9002\u91cf\u7684\u743c\u8102\u7cd6\u7c89\u672b\uff0c\u52a0\u5165\u7f13\u51b2\u6db2\u4e2d\u3002\n3. **\u52a0\u70ed\u6eb6\u89e3**\uff1a\u5c06\u743c\u8102\u7cd6\u6eb6\u6db2\u52a0\u70ed\u81f3\u6cb8\u817e\uff0c\u6301\u7eed\u6405\u62cc\uff0c\u76f4\u5230\u743c\u8102\u7cd6\u5b8c\u5168\u6eb6\u89e3\uff0c\u6eb6\u6db2\u900f\u660e\u4e3a\u6b62\u3002\n4. **\u51b7\u5374\u81f3\u9002\u5f53\u6e29\u5ea6**\uff1a\u5c06\u70ed\u6eb6\u6db2\u51b7\u5374\u81f3\u7ea660\u00b0C\uff0c\u4ee5\u4fbf\u5728\u5012\u6a21\u65f6\u4e0d\u4f1a\u635f\u574f\u6a21\u5177\u3002\n5. **\u5012\u6a21**\uff1a\u5c06\u51b7\u5374\u540e\u7684\u6eb6\u6db2\u5012\u5165\u51dd\u80f6\u6a21\u5177\u4e2d\uff0c\u63d2\u5165\u68b3\u5b50\u5f62\u6210\u6837\u54c1\u5b54\u3002\n6. **\u51dd\u56fa**\uff1a\u5728\u5ba4\u6e29\u6216\u4f4e\u6e29\u73af\u5883\u4e2d\u653e\u7f6e\u4e00\u6bb5\u65f6\u95f4\uff0c\u76f4\u5230\u51dd\u80f6\u5b8c\u5168\u51dd\u56fa\u3002\n7. **\u53d6\u51fa\u68b3\u5b50**\uff1a\u5c0f\u5fc3\u53d6\u51fa\u68b3\u5b50\uff0c\u51dd\u80f6\u5373\u53ef\u7528\u4e8e\u7535\u6cf3\u5b9e\u9a8c\u3002\n\n### \u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\n1. **\u914d\u5236\u51dd\u80f6\u6eb6\u6db2**\uff1a\u6839\u636e\u6240\u9700\u7684\u6d53\u5ea6\uff08\u5e38\u75285%\u523015%\u4e4b\u95f4\uff09\uff0c\u914d\u5236\u4e19\u70ef\u9170\u80fa/\u53cc\u4e19\u70ef\u9170\u80fa\u6eb6\u6db2\u3002\u4f7f\u7528\u9002\u5f53\u7684\u7f13\u51b2\u6db2\uff08\u5982Tris-HCl\uff09\u3002\n2. **\u53bb\u6c14**\uff1a\u5c06\u6eb6\u6db2\u7f6e\u4e8e\u771f\u7a7a\u4e2d\u53bb\u6c14\uff0c\u4ee5\u9664\u53bb\u6eb6\u89e3\u7684\u6c27\u6c14\uff0c\u9632\u6b62\u805a\u5408\u53cd\u5e94\u53d7\u5230\u5e72\u6270\u3002\n3. **\u52a0\u5165\u50ac\u5316\u5242**\uff1a\u52a0\u5165\u8fc7\u786b\u9178\u94f5\uff08APS\uff09\u548c\u56db\u7532\u57fa\u4e59\u4e8c\u80fa\uff08TEMED\uff09\u4f5c\u4e3a\u805a\u5408\u50ac\u5316\u5242\u3002\n4. **\u5feb\u901f\u5012\u6a21**\uff1a\u5c06\u6df7\u5408\u540e\u7684\u6eb6\u6db2\u8fc5\u901f\u5012\u5165\u6a21\u5177\u4e2d\uff0c\u63d2\u5165\u68b3\u5b50\u3002\n5. **\u805a\u5408**\uff1a\u5728\u5ba4\u6e29\u4e0b\u9759\u7f6e15-30\u5206\u949f\uff0c\u76f4\u5230\u51dd\u80f6\u5b8c\u5168\u805a\u5408\u3002\n6. **\u53d6\u51fa\u68b3\u5b50**\uff1a\u5c0f\u5fc3\u53d6\u51fa\u68b3\u5b50\uff0c\u51dd\u80f6\u5373\u5236\u4f5c\u5b8c\u6210\u3002\n\n\u8fd9\u4e24\u79cd\u51dd\u80f6\u7684\u5236\u5907\u8fc7\u7a0b\u90fd\u6709\u4e00\u4e9b\u9700\u8981\u6ce8\u610f\u7684\u7ec6\u8282\uff0c\u4f8b\u5982\u6eb6\u6db2\u7684\u53bb\u6c14\u3001\u52a0\u70ed\u6eb6\u89e3\u7684\u5747\u5300\u6027\u3001\u4ee5\u53ca\u5728\u51dd\u80f6\u6210\u578b\u8fc7\u7a0b\u4e2d\u907f\u514d\u6c14\u6ce1\u7684\u4ea7\u751f\u7b49\u301011\u2020source\u3011\u301012\u2020source\u3011\u301013\u2020source\u3011\u3002", "model": "gpt-4o-all", "timestamp": 1716008460, "done": true}, "968cfd61-75a4-490a-8e51-7fe9d07a2668": {"parentId": "680ced3e-1e3b-4c68-af25-8fd55dc74435", "id": "968cfd61-75a4-490a-8e51-7fe9d07a2668", "childrenIds": [], "role": "assistant", "content": "#### \u51dd\u80f6\u7535\u6cf3\u4e2d\u51dd\u80f6\u7684\u5236\u5907\u6b65\u9aa4\n\n1. \u51c6\u5907\u51dd\u80f6\u5236\u5907\u6240\u9700\u7684\u8bd5\u5242\u548c\u5668\u6750\uff0c\u5305\u62ec\u4e19\u70ef\u9170\u80fa\u3001\u7532\u53c9\u53cc\u4e19\u70ef\u9170\u80fa\uff08\u4ea4\u8054\u5242\uff09\u3001\u7f13\u51b2\u6db2\u3001\u8fc7\u786b\u9178\u94f5\uff08\u5f15\u53d1\u5242\uff09\u3001TEMED\uff08\u50ac\u5316\u5242\uff09\u7b49\uff0c\u4ee5\u53ca\u73bb\u7483\u677f\u3001\u68b3\u5b50\u3001\u7535\u6cf3\u69fd\u7b49\u5668\u6750\u3002\n\n2. \u6839\u636e\u5b9e\u9a8c\u9700\u8981\uff0c\u9009\u62e9\u5408\u9002\u7684\u4e19\u70ef\u9170\u80fa\u6d53\u5ea6\uff08\u901a\u5e38\u4e3a5-15%\uff09\uff0c\u5e76\u8ba1\u7b97\u914d\u5236\u51dd\u80f6\u6240\u9700\u7684\u5404\u79cd\u8bd5\u5242\u7528\u91cf\u3002\n\n3. \u5c06\u8ba1\u7b97\u597d\u7684\u4e19\u70ef\u9170\u80fa\u3001\u7532\u53c9\u53cc\u4e19\u70ef\u9170\u80fa\u3001\u7f13\u51b2\u6db2\u7b49\u52a0\u5165\u70e7\u676f\u4e2d\uff0c\u7528\u84b8\u998f\u6c34\u5b9a\u5bb9\uff0c\u6df7\u5300\u3002\n\n4. \u52a0\u5165\u8fc7\u786b\u9178\u94f5\u548cTEMED\uff0c\u5feb\u901f\u6df7\u5300\uff0c\u7acb\u5373\u5012\u5165\u5df2\u7ec4\u88c5\u597d\u7684\u73bb\u7483\u677f\u4e2d\uff0c\u63d2\u5165\u68b3\u5b50\uff0c\u9759\u7f6e20-30\u5206\u949f\uff0c\u5f85\u51dd\u80f6\u5145\u5206\u805a\u5408\u3002\n\n5. \u51dd\u80f6\u805a\u5408\u540e\uff0c\u53d6\u51fa\u68b3\u5b50\uff0c\u7528\u7f13\u51b2\u6db2\u6da6\u6d17\u51dd\u80f6\u8868\u9762\u7684\u6837\u54c1\u5b54\uff0c\u9664\u53bb\u672a\u805a\u5408\u7684\u4e19\u70ef\u9170\u80fa\u3002\n\n6. \u5c06\u51dd\u80f6\u677f\u653e\u5165\u7535\u6cf3\u69fd\uff0c\u52a0\u5165\u7f13\u51b2\u6db2\uff0c\u5373\u53ef\u8fdb\u884c\u6837\u54c1\u7684\u4e0a\u6837\u548c\u7535\u6cf3\u3002\n\n#### \u6ce8\u610f\u4e8b\u9879\n\n- \u914d\u5236\u51dd\u80f6\u6eb6\u6db2\u65f6\u8981\u51c6\u786e\u79f0\u91cf\uff0c\u5e76\u5145\u5206\u6df7\u5300\uff0c\u907f\u514d\u5c40\u90e8\u805a\u5408\u3002\n- **\u52a0\u5165\u8fc7\u786b\u9178\u94f5\u548cTEMED\u540e\u8981\u5feb\u901f\u64cd\u4f5c\uff0c\u5426\u5219\u51dd\u80f6\u53ef\u80fd\u5728\u73bb\u7483\u677f\u5916\u805a\u5408**\u3002\n- \u63d2\u5165\u68b3\u5b50\u65f6\u8981\u907f\u514d\u4ea7\u751f\u6c14\u6ce1\uff0c\u5f71\u54cd\u6837\u54c1\u5b54\u7684\u5f62\u6210\u3002\n- \u7535\u6cf3\u7f13\u51b2\u6db2\u7684\u914d\u5236\u8981\u51c6\u786e\uff0cpH\u503c\u8981\u5408\u9002\uff0c\u4ee5\u4fdd\u8bc1\u7535\u6cf3\u8fc7\u7a0b\u7684\u7a33\u5b9a\u6027\u548c\u91cd\u590d\u6027\u3002\n\n\u4ee5\u4e0a\u5c31\u662f\u5b9e\u9a8c\u5ba4\u4e2d\u51dd\u80f6\u7535\u6cf3\u5e38\u7528\u7684\u51dd\u80f6\u5236\u5907\u65b9\u6cd5\u548c\u6ce8\u610f\u4e8b\u9879\uff0c\u64cd\u4f5c\u65f6\u6ce8\u610f\u7ec6\u8282\uff0c\u4e25\u683c\u63a7\u5236\u5404\u6b65\u9aa4\uff0c\u53ef\u4ee5\u5f97\u5230\u8d28\u91cf\u7a33\u5b9a\u3001\u5206\u8fa8\u7387\u826f\u597d\u7684\u51dd\u80f6\uff0c\u4e3a\u540e\u7eed\u7684\u7535\u6cf3\u5b9e\u9a8c\u5960\u5b9a\u57fa\u7840\u3002", "model": "claude-3-opus-20240229", "timestamp": 1716008460, "done": true}, "9a251bdf-d538-447c-a805-1ea6998a90c7": {"id": "9a251bdf-d538-447c-a805-1ea6998a90c7", "parentId": null, "childrenIds": ["bf74c6be-a126-4897-b677-c5cbba35bb22", "74c66e51-93a7-483e-a3f7-3ccbe9f1d14f", "c4c69f2c-a864-4bbc-a1c3-1aaaa4c4222d"], "role": "user", "content": "\u5b9e\u9a8c\u5ba4\u4e2d\u68c0\u6d4bdna\u7684\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u5e94\u8be5\u600e\u6837\u64cd\u4f5c\uff1f\u641c\u7d22\u540e\u7528\u4e2d\u6587\u56de\u7b54\uff0c\u5c3d\u53ef\u80fd\u8be6\u7ec6"}, "bf74c6be-a126-4897-b677-c5cbba35bb22": {"parentId": "9a251bdf-d538-447c-a805-1ea6998a90c7", "id": "bf74c6be-a126-4897-b677-c5cbba35bb22", "childrenIds": [], "role": "assistant", "content": "\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u662f\u4e00\u79cd\u5206\u79bb\u548c\u5206\u6790DNA\u7247\u6bb5\u5927\u5c0f\u7684\u5b9e\u9a8c\u65b9\u6cd5\u3002\u4ee5\u4e0b\u662f\u8fdb\u884c\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u7684\u8be6\u7ec6\u64cd\u4f5c\u6b65\u9aa4\uff1a\n\n1. **\u51c6\u5907\u51dd\u80f6**: \u9996\u5148\uff0c\u6839\u636e\u9700\u8981\u5206\u79bb\u7684DNA\u7247\u6bb5\u5927\u5c0f\uff0c\u9009\u62e9\u5408\u9002\u6d53\u5ea6\u7684\u743c\u8102\u7cd6\u3002\u5e38\u7528\u7684\u743c\u8102\u7cd6\u6d53\u5ea6\u8303\u56f4\u4ece0.7%\u52302%\u4e0d\u7b49\uff0c\u4ee5\u5206\u79bb\u4e0d\u540c\u5927\u5c0f\u7684DNA\u7247\u6bb5\u3002\u5c06\u743c\u8102\u7cd6\u7c89\u672b\u79f0\u91cf\u540e\uff0c\u52a0\u5165\u9002\u91cf\u76841x TAE\u6216TBE\u7f13\u51b2\u6db2\uff08\u4e24\u79cd\u6700\u5e38\u89c1\u7684\u7535\u6cf3\u7f13\u51b2\u6db2\uff09\uff0c\u7528\u5fae\u6ce2\u7089\u52a0\u70ed\u81f3\u743c\u8102\u7cd6\u5b8c\u5168\u878d\u5316\u3002\n\n2. **\u5236\u4f5c\u51dd\u80f6**: \u5c06\u878d\u5316\u7684\u743c\u8102\u7cd6\u6eb6\u6db2\u51b7\u5374\u81f360\u00b0C\u5de6\u53f3\uff0c\u7136\u540e\u5012\u5165\u5df2\u7ecf\u56fa\u5b9a\u597d\u68b3\u5b50\u7684\u7535\u6cf3\u69fd\u4e2d\uff0c\u5f85\u51dd\u80f6\u5b8c\u5168\u51dd\u56fa\uff08\u5927\u7ea6\u9700\u898130\u5206\u949f\uff09\u3002\u51dd\u56fa\u540e\u5c0f\u5fc3\u62d4\u51fa\u68b3\u5b50\uff0c\u5f62\u6210\u6837\u54c1\u5b54\u3002\n\n3. **\u51c6\u5907\u6837\u54c1**: \u5c06DNA\u6837\u54c1\u4e0e\u4e0a\u6837\u7f13\u51b2\u6db2\uff08loading buffer\uff09\u548c\u67d3\u6599\uff08\u5982\u6eb4\u5316\u4e59\u952d\u6216SYBR Safe\uff09\u6df7\u5408\uff0c\u4f7f\u6837\u54c1\u5728\u7d2b\u5916\u5149\u4e0b\u53ef\u89c1\u3002\n\n4. **\u52a0\u8f7d\u6837\u54c1**: \u5c06\u6df7\u5408\u597d\u7684\u6837\u54c1\u7f13\u6162\u52a0\u5165\u5230\u51dd\u80f6\u7684\u6837\u54c1\u5b54\u4e2d\u3002\n\n5. **\u7535\u6cf3**: \u5c06\u7535\u6cf3\u69fd\u7f6e\u4e8e\u7535\u6e90\u88c5\u7f6e\u4e2d\uff0c\u8bbe\u7f6e\u9002\u5f53\u7684\u7535\u538b\uff08\u4e00\u822c\u4e0d\u8d85\u8fc75V/cm\uff09\uff0c\u5f00\u542f\u7535\u6e90\u3002DNA\u7531\u4e8e\u5e26\u8d1f\u7535\uff0c\u4f1a\u4ece\u8d1f\u6781\uff08\u52a0\u6837\u7aef\uff09\u5411\u6b63\u6781\u65b9\u5411\u8fc1\u79fb\u3002\n\n6. **\u89c2\u5bdf\u548c\u8bb0\u5f55**: \u7535\u6cf3\u5b8c\u6210\u540e\uff0c\u4f7f\u7528\u7d2b\u5916\u900f\u89c6\u4eea\u89c2\u5bdf\u548c\u8bb0\u5f55DNA\u6761\u5e26\u7684\u8fc1\u79fb\u60c5\u51b5\u3002\u6bd4\u8f83\u5df2\u77e5\u5927\u5c0f\u7684DNA\u6807\u8bb0\uff08marker\uff09\uff0c\u53ef\u4ee5\u4f30\u8ba1\u672a\u77e5\u6837\u54c1\u7684\u5927\u5c0f\u3002\n\n7. **\u56de\u6536\u548c\u5206\u6790**: \u5982\u9700\u8981\uff0c\u53ef\u4ece\u51dd\u80f6\u4e2d\u5207\u5272\u611f\u5174\u8da3\u7684DNA\u6761\u5e26\u8fdb\u884c\u8fdb\u4e00\u6b65\u7684\u56de\u6536\u6216\u5206\u6790\u3002\n\n\u8fd9\u4e9b\u6b65\u9aa4\u9700\u8981\u5728\u907f\u514d\u6838\u9178\u9176\u6c61\u67d3\u7684\u6761\u4ef6\u4e0b\u8fdb\u884c\uff0c\u4ee5\u4fdd\u8bc1\u5b9e\u9a8c\u7ed3\u679c\u7684\u51c6\u786e\u6027\u3002\u6b64\u5916\uff0c\u5b9e\u9a8c\u4e2d\u4f7f\u7528\u7684\u6240\u6709\u5de5\u5177\u548c\u7f13\u51b2\u6db2\u90fd\u5e94\u4fdd\u6301\u65e0\u83cc\u301011\u2020source\u3011\u301012\u2020source\u3011\u301013\u2020source\u3011\u3002", "model": "gpt-4-all", "timestamp": 1716014489, "done": true}, "74c66e51-93a7-483e-a3f7-3ccbe9f1d14f": {"parentId": "9a251bdf-d538-447c-a805-1ea6998a90c7", "id": "74c66e51-93a7-483e-a3f7-3ccbe9f1d14f", "childrenIds": [], "role": "assistant", "content": "\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u662f\u4e00\u79cd\u7528\u4e8e\u5206\u79bb\u548c\u5206\u6790DNA\u7247\u6bb5\u7684\u5e38\u89c1\u6280\u672f\u3002\u4ee5\u4e0b\u662f\u8be6\u7ec6\u7684\u64cd\u4f5c\u6b65\u9aa4\uff1a\n\n1. **\u51c6\u5907\u5de5\u4f5c**\uff1a\n   - \u6e05\u6d17\u5236\u80f6\u5de5\u5177\u5e76\u51c6\u5907\u5236\u80f6\u5e73\u677f\uff0c\u7528\u743c\u8102\u5bc6\u5c01\u6a21\u5177\u8fb9\u7f18\uff0c\u5e76\u63d2\u5165\u68b3\u5b50\u3002\n   - \u914d\u5236\u7535\u6cf3\u7f13\u51b2\u6db2\uff08\u5982TAE\u6216TBE\uff09\uff0c\u7528\u84b8\u998f\u6c34\u7a00\u91ca\u81f3\u6240\u9700\u6d53\u5ea6\u30107\u2020source\u3011\u30108\u2020source\u3011\u3002\n\n2. **\u5236\u5907\u743c\u8102\u7cd6\u51dd\u80f6**\uff1a\n   - \u79f0\u53d6\u9002\u91cf\u7684\u743c\u8102\u7cd6\u7c89\u672b\uff0c\u6839\u636e\u6240\u9700\u6d53\u5ea6\u52a0\u5165\u5230\u9525\u5f62\u74f6\u4e2d\uff0c\u52a0\u5165\u7f13\u51b2\u6db2\uff08\u59820.5\u00d7TBE\uff09\u30108\u2020source\u3011\u3002\n   - \u5728\u5fae\u6ce2\u7089\u4e2d\u52a0\u70ed\u81f3\u743c\u8102\u7cd6\u5b8c\u5168\u6eb6\u89e3\uff0c\u51b7\u5374\u81f360\u2103\u5de6\u53f3\uff0c\u52a0\u5165\u9002\u91cf\u7684\u6eb4\u5316\u4e59\u952d\uff08EB\uff09\u81f30.5\u00b5g/ml\u30108\u2020source\u3011\u30109\u2020source\u3011\u3002\n   - \u5c06\u6eb6\u89e3\u597d\u7684\u743c\u8102\u7cd6\u5012\u5165\u5236\u80f6\u677f\u4e2d\uff0c\u5f85\u5176\u51dd\u56fa\u3002\u51dd\u56fa\u540e\u5c0f\u5fc3\u62d4\u51fa\u68b3\u5b50\u30108\u2020source\u3011\u3002\n\n3. **\u7535\u6cf3\u69fd\u51c6\u5907**\uff1a\n   - \u5c06\u51dd\u56fa\u597d\u7684\u743c\u8102\u7cd6\u51dd\u80f6\u653e\u5165\u7535\u6cf3\u69fd\uff0c\u52a0\u5165\u7f13\u51b2\u6db2\u81f3\u6db2\u9762\u521a\u597d\u8986\u76d6\u51dd\u80f6\u8868\u9762\u30107\u2020source\u3011\u3002\n\n4. **\u4e0a\u6837\u548c\u7535\u6cf3**\uff1a\n   - \u5c06DNA\u6837\u54c1\u4e0e\u52a0\u6837\u7f13\u51b2\u6db2\uff08\u59826\u00d7loading buffer\uff09\u6df7\u5408\uff0c\u6162\u6162\u52a0\u5165\u51dd\u80f6\u5b54\u4e2d\u30108\u2020source\u3011\u30109\u2020source\u3011\u3002\n   - \u63a5\u901a\u7535\u6e90\uff0c\u8bbe\u7f6e\u9002\u5f53\u7684\u7535\u538b\uff08\u901a\u5e3840-50V\uff09\uff0cDNA\u5c06\u5728\u7535\u573a\u4f5c\u7528\u4e0b\u4ece\u8d1f\u6781\u5411\u6b63\u6781\u79fb\u52a8\u30107\u2020source\u3011\u3002\n\n5. **\u67d3\u8272\u548c\u89c2\u5bdf**\uff1a\n   - \u7535\u6cf3\u7ed3\u675f\u540e\uff0c\u5c06\u51dd\u80f6\u5728\u542b0.5\u00b5g/ml\u6eb4\u5316\u4e59\u952d\u7684\u7f13\u51b2\u6db2\u4e2d\u67d3\u827210-30\u5206\u949f\u3002\n   - \u7528\u7d2b\u5916\u900f\u5c04\u4eea\u89c2\u5bdfDNA\u6761\u5e26\uff0c\u5e76\u4e0e\u5206\u5b50\u91cf\u6807\u51c6\uff08\u59821kb Marker\uff09\u8fdb\u884c\u6bd4\u8f83\u30108\u2020source\u3011\u30109\u2020source\u3011\u3002\n\n6. **DNA\u56de\u6536\uff08\u53ef\u9009\uff09**\uff1a\n   - \u9700\u8981\u56de\u6536\u7279\u5b9aDNA\u7247\u6bb5\u65f6\uff0c\u53ef\u4ee5\u4f7f\u7528\u4f4e\u7194\u70b9\u743c\u8102\u7cd6\u6cd5\u6216\u8bd5\u5242\u76d2\u7b49\u65b9\u6cd5\u8fdb\u884c\u56de\u6536\u30107\u2020source\u3011\u3002\n\n\u8fd9\u4e9b\u6b65\u9aa4\u6d89\u53ca\u7684\u5173\u952e\u8981\u70b9\u5305\u62ec\uff1a\u7cbe\u786e\u7684\u7f13\u51b2\u6db2\u914d\u5236\u3001\u9002\u5f53\u7684\u7535\u6cf3\u6761\u4ef6\u8bbe\u7f6e\u4ee5\u53ca\u5b89\u5168\u6709\u6548\u7684\u67d3\u8272\u65b9\u6cd5\uff0c\u4ee5\u786e\u4fdd\u5b9e\u9a8c\u7ed3\u679c\u7684\u51c6\u786e\u6027\u548c\u91cd\u590d\u6027\u3002", "model": "gpt-4o-all", "timestamp": 1716014489, "done": true}, "c4c69f2c-a864-4bbc-a1c3-1aaaa4c4222d": {"parentId": "9a251bdf-d538-447c-a805-1ea6998a90c7", "id": "c4c69f2c-a864-4bbc-a1c3-1aaaa4c4222d", "childrenIds": [], "role": "assistant", "content": "#### \u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u64cd\u4f5c\u6b65\u9aa4\n\n1. **\u5236\u5907\u743c\u8102\u7cd6\u51dd\u80f6**\uff1a\u6839\u636eDNA\u7247\u6bb5\u5927\u5c0f\u9009\u62e9\u5408\u9002\u6d53\u5ea6\u7684\u743c\u8102\u7cd6\uff08\u901a\u5e38\u4e3a0.7%-2%\uff09\uff0c\u79f0\u53d6\u76f8\u5e94\u8d28\u91cf\u7684\u743c\u8102\u7cd6\u7c89\u672b\uff0c\u52a0\u5165\u9002\u91cf\u76841\u00d7TAE\u62161\u00d7TBE\u7f13\u51b2\u6db2\uff0c\u52a0\u70ed\u6eb6\u89e3\u81f3\u6f84\u6e05\u900f\u660e\uff0c\u51b7\u5374\u81f3\u7ea650-60\u2103\u3002\n\n2. **\u5236\u80f6**\uff1a\u5c06\u51b7\u5374\u540e\u7684\u743c\u8102\u7cd6\u6eb6\u6db2\u5012\u5165\u5df2\u7ec4\u88c5\u597d\u7684\u51dd\u80f6\u6a21\u5177\u4e2d\uff0c\u63d2\u5165\u68b3\u5b50\u5f62\u6210\u6837\u54c1\u5b54\uff0c\u9759\u7f6e\u81f3\u5b8c\u5168\u51dd\u56fa\uff08\u7ea630\u5206\u949f\uff09\u3002\n\n3. **\u4e0a\u6837**\uff1a\u53d6\u51fa\u68b3\u5b50\uff0c\u5c06\u51dd\u80f6\u8fde\u540c\u6258\u76d8\u653e\u5165\u7535\u6cf3\u69fd\uff0c\u52a0\u5165\u8db3\u91cf\u76841\u00d7TAE\u62161\u00d7TBE\u7f13\u51b2\u6db2\u6ca1\u8fc7\u51dd\u80f6\u8868\u9762\u3002\u5c06\u5f85\u6d4bDNA\u6837\u54c1\u4e0e6\u00d7\u4e0a\u6837\u7f13\u51b2\u6db2\u63095:1\u6df7\u5300\uff0c\u5c0f\u5fc3\u52a0\u5165\u6837\u54c1\u5b54\u4e2d\u3002\u540c\u65f6\u52a0\u5165DNA Marker\u4f5c\u4e3a\u5206\u5b50\u91cf\u6807\u51c6\u3002\n\n4. **\u7535\u6cf3**\uff1a\u76d6\u4e0a\u7535\u6cf3\u69fd\u76d6\u5b50\uff0c\u8fde\u63a5\u7535\u6e90\uff0c\u6839\u636e\u51dd\u80f6\u5927\u5c0f\u548c\u6d53\u5ea6\u8bbe\u7f6e\u5408\u9002\u7684\u7535\u538b\uff08\u901a\u5e38\u4e3a5-10 V/cm\uff09\uff0c\u7535\u6cf3\u76f4\u81f3\u6eb4\u915a\u84dd\u6307\u793a\u5242\u8fc1\u79fb\u81f3\u51dd\u80f62/3\u5904\u3002\n\n5. **\u67d3\u8272\u4e0e\u89c2\u5bdf**\uff1a\u7535\u6cf3\u7ed3\u675f\u540e\uff0c\u5c06\u51dd\u80f6\u7f6e\u4e8e\u542b\u6709\u6838\u9178\u67d3\u6599\uff08\u5982\u6eb4\u5316\u4e59\u952d\uff09\u7684\u67d3\u8272\u7f13\u51b2\u6db2\u4e2d\u67d3\u827210-30\u5206\u949f\u3002\u7136\u540e\u7528\u6c34\u7b80\u5355\u8131\u8272\uff0c\u5728\u7d2b\u5916\u706f\u4e0b\u89c2\u5bdf\u62cd\u7167\u3002\n\n6. **\u7ed3\u679c\u5206\u6790**\uff1a\u6839\u636eDNA Marker\u63a8\u6d4b\u76ee\u7684\u6761\u5e26\u5927\u5c0f\uff0c\u521d\u6b65\u5224\u65ad\u5b9e\u9a8c\u7ed3\u679c\u3002\u5982\u6709\u5fc5\u8981\u53ef\u5207\u80f6\u56de\u6536\u76ee\u7684\u7247\u6bb5\u8fdb\u884c\u540e\u7eed\u5b9e\u9a8c\u3002\n\n\u6ce8\u610f\u4e8b\u9879\uff1a\n- \u914d\u5236\u7f13\u51b2\u6db2\u548c\u6eb6\u6db2\u65f6\u5e94\u51c6\u786e\u79f0\u91cf\u3002\n- \u52a0\u6837\u65f6\u907f\u514d\u6837\u54c1\u6ea2\u51fa\u6c61\u67d3\u5176\u4ed6\u6cf3\u9053\u3002\n- \u7535\u6cf3\u65f6\u6ce8\u610f\u7f13\u51b2\u6db2\u6db2\u9762\uff0c\u5fc5\u8981\u65f6\u53ef\u8865\u5145\u3002\n- \u6eb4\u5316\u4e59\u952d\u6709\u81f4\u7a81\u53d8\u6027\uff0c\u67d3\u8272\u548c\u89c2\u5bdf\u65f6\u5e94\u6234\u624b\u5957\u5e76\u5728\u901a\u98ce\u6a71\u4e2d\u8fdb\u884c\uff0c\u7d2b\u5916\u706f\u4e0b\u89c2\u5bdf\u65f6\u95f4\u4e0d\u5b9c\u8fc7\u957f\u3002\n\n\u4ee5\u4e0a\u5c31\u662f\u5b9e\u9a8c\u5ba4\u4e2d\u8fdb\u884c\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3DNA\u68c0\u6d4b\u7684\u57fa\u672c\u64cd\u4f5c\u6d41\u7a0b\u548c\u6ce8\u610f\u4e8b\u9879\uff0c\u5177\u4f53\u5b9e\u9a8c\u6761\u4ef6\u53ef\u6839\u636e\u5b9e\u9645\u60c5\u51b5\u8fdb\u884c\u4f18\u5316\u3002", "model": "claude-3-opus-20240229", "timestamp": 1716014489, "done": true}, "a25669df-a668-4daf-aa8e-f31a72396f8c": {"id": "a25669df-a668-4daf-aa8e-f31a72396f8c", "parentId": null, "childrenIds": ["178fcf9e-2e63-4908-9c4c-e297aca20a25", "dd76f84f-e67b-49e8-b970-3b00e408d62a", "a513174a-2d5b-40b2-84e5-cc1b297ef4fc"], "role": "user", "content": "\u5b9e\u9a8c\u5ba4\u4e2d\u68c0\u6d4bdna\u7684\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u5e94\u8be5\u600e\u6837\u64cd\u4f5c\uff1f\u641c\u7d22\u540e\u7528\u4e2d\u6587\u56de\u7b54\uff0c\u5c3d\u53ef\u80fd\u8be6\u7ec6\uff0c\u5e2e\u6211\u5f62\u6210Markdown\u683c\u5f0f\u7684\u7b14\u8bb0"}, "178fcf9e-2e63-4908-9c4c-e297aca20a25": {"parentId": "a25669df-a668-4daf-aa8e-f31a72396f8c", "id": "178fcf9e-2e63-4908-9c4c-e297aca20a25", "childrenIds": [], "role": "assistant", "content": "\u4ee5\u4e0b\u662f\u4e00\u4efd\u8be6\u7ec6\u7684\u5b9e\u9a8c\u6b65\u9aa4\u7b14\u8bb0\uff0c\u7528\u4e8e\u6307\u5bfc\u4f60\u5b8c\u6210\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u7684\u64cd\u4f5c\uff0c\u5e2e\u52a9\u5206\u79bb\u548c\u9274\u5b9aDNA\u6837\u54c1\uff1a\n\n---\n\n### \u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u5b9e\u9a8c\u6b65\u9aa4\n\n#### \u6750\u6599\u548c\u8bd5\u5242\u51c6\u5907\n1. **\u743c\u8102\u7cd6**\uff1a\u6839\u636e\u9700\u8981\u5206\u79bb\u7684DNA\u7247\u6bb5\u5927\u5c0f\u9009\u62e9\u5408\u9002\u7684\u743c\u8102\u7cd6\u6d53\u5ea6\u3002\n2. **1\u00d7TAE\u62161\u00d7TBE\u7535\u6cf3\u7f13\u51b2\u6db2**\uff1a\u6839\u636e\u5b9e\u9a8c\u9700\u6c42\u51c6\u5907\u3002\n3. **DNA\u6837\u54c1**\uff1a\u51c6\u5907\u5f85\u5206\u79bb\u7684DNA\u3002\n4. **\u67d3\u6599**\uff1a\u5982EB\uff08\u6eb4\u5316\u4e59\u952d\uff09\u7528\u4e8e\u67d3\u8272DNA\u3002\n\n#### \u5236\u5907\u743c\u8102\u7cd6\u51dd\u80f6\n1. \u6e05\u6d17\u5236\u80f6\u5de5\u5177\u548c\u51dd\u80f6\u5e73\u677f\u3002\n2. \u7cbe\u786e\u79f0\u53d6\u743c\u8102\u7cd6\u7c89\u672b\u5e76\u52a0\u5165\u9002\u91cf\u7684\u7535\u6cf3\u7f13\u51b2\u6db2\u3002\n3. \u5c06\u6df7\u5408\u7269\u5728\u5fae\u6ce2\u7089\u4e2d\u52a0\u70ed\u81f3\u5b8c\u5168\u6eb6\u89e3\u3002\n4. \u51b7\u5374\u81f360\u00b0C\u540e\uff0c\u5012\u5165\u51c6\u5907\u597d\u7684\u5e73\u677f\u4e2d\uff0c\u5e76\u63d2\u5165\u68b3\u5b50\u5f62\u6210\u6837\u54c1\u5b54\u3002\n5. \u5f85\u51dd\u80f6\u51dd\u56fa\u540e\uff08\u7ea630-40\u5206\u949f\uff09\uff0c\u5c0f\u5fc3\u79fb\u9664\u68b3\u5b50\u3002\n\n#### \u4e0a\u6837\u548c\u7535\u6cf3\n1. \u5728\u7535\u6cf3\u69fd\u4e2d\u52a0\u5165\u8db3\u591f\u7684\u7535\u6cf3\u7f13\u51b2\u6db2\uff0c\u8986\u76d6\u51dd\u80f6\u8868\u9762\u3002\n2. \u5c06DNA\u6837\u54c1\u4e0e\u4e0a\u6837\u7f13\u51b2\u6db2\u6df7\u5408\u540e\uff0c\u5c0f\u5fc3\u52a0\u5165\u51dd\u80f6\u7684\u6837\u54c1\u5b54\u4e2d\u3002\n3. \u63a5\u901a\u7535\u6e90\uff0c\u8bbe\u7f6e\u5408\u9002\u7684\u7535\u538b\u548c\u65f6\u95f4\uff0c\u901a\u5e38\u4e3a40-50\u4f0f\uff0c\u7535\u6cf3\u65f6\u95f4\u4e0d\u8d85\u8fc740\u5206\u949f\u3002\n\n#### \u67d3\u8272\u548c\u89c2\u5bdf\n1. \u7535\u6cf3\u7ed3\u675f\u540e\uff0c\u5c06\u51dd\u80f6\u7f6e\u4e8e\u542b\u6709EB\u7684\u67d3\u8272\u6db2\u4e2d\uff0c\u6d78\u6ce110-30\u5206\u949f\u3002\n2. \u4f7f\u7528\u7d2b\u5916\u5149\u6e90\u89c2\u5bdf\u548c\u62cd\u6444DNA\u6761\u5e26\u3002\n\n#### \u6ce8\u610f\u4e8b\u9879\n- \u786e\u4fdd\u4f7f\u7528\u7684\u743c\u8102\u7cd6\u548c\u7f13\u51b2\u6db2\u914d\u6bd4\u51c6\u786e\uff0c\u4ee5\u907f\u514d\u5f71\u54cd\u5206\u79bb\u6548\u679c\u3002\n- \u64cd\u4f5c\u8fc7\u7a0b\u4e2d\u5e94\u907f\u514d\u6ce1\u6cab\u7684\u4ea7\u751f\uff0c\u4ee5\u514d\u5f71\u54cd\u6837\u54c1\u7684\u52a0\u8f7d\u548c\u7535\u6cf3\u6548\u679c\u3002\n- \u7535\u6cf3\u8fc7\u7a0b\u4e2d\u6ce8\u610f\u7535\u538b\u548c\u65f6\u95f4\u7684\u63a7\u5236\uff0c\u9632\u6b62DNA\u6837\u54c1\u8fc7\u5ea6\u8fc1\u79fb\u6216\u4e0d\u8db3\u3002\n\n#### \u6e05\u6d01\u548c\u4fdd\u517b\n- \u5b9e\u9a8c\u540e\u6e05\u6d01\u6240\u6709\u8bbe\u5907\u548c\u5de5\u5177\uff0c\u786e\u4fdd\u6ca1\u6709\u6b8b\u7559\u7684\u51dd\u80f6\u6216\u67d3\u6599\u3002\n- \u50a8\u5b58\u743c\u8102\u7cd6\u51dd\u80f6\u548c\u7f13\u51b2\u6db2\u4e8e\u63a8\u8350\u7684\u6761\u4ef6\u4e0b\uff0c\u907f\u514d\u6c61\u67d3\u548c\u964d\u89e3\u3002\n\n---\n\n\u8fd9\u4efd\u64cd\u4f5c\u624b\u518c\u5e94\u6db5\u76d6\u4ece\u51dd\u80f6\u5236\u5907\u5230DNA\u7684\u89c2\u5bdf\u5168\u8fc7\u7a0b\u7684\u8be6\u7ec6\u6b65\u9aa4\uff0c\u9002\u5408\u521d\u5b66\u8005\u548c\u9700\u8981\u590d\u4e60\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u6280\u672f\u7684\u7814\u7a76\u4eba\u5458\u3002\u5e0c\u671b\u8fd9\u53ef\u4ee5\u5e2e\u52a9\u4f60\u5728\u5b9e\u9a8c\u4e2d\u53d6\u5f97\u597d\u7684\u7ed3\u679c\uff01\u30107\u2020source\u3011\u30108\u2020source\u3011\u30109\u2020source\u3011.", "model": "gpt-4-all", "timestamp": 1716015232, "done": true}, "dd76f84f-e67b-49e8-b970-3b00e408d62a": {"parentId": "a25669df-a668-4daf-aa8e-f31a72396f8c", "id": "dd76f84f-e67b-49e8-b970-3b00e408d62a", "childrenIds": [], "role": "assistant", "content": "### \u5b9e\u9a8c\u5ba4\u4e2d\u68c0\u6d4bDNA\u7684\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u64cd\u4f5c\u6b65\u9aa4\n\n#### \u6750\u6599\u548c\u8bd5\u5242\u51c6\u5907\n- **\u6750\u6599**: \n  - DNA\u6837\u54c1\n  - 1kb DNA\u5206\u5b50\u91cf\u6807\u51c6\uff08Marker\uff09\n- **\u8bd5\u5242**: \n  - \u743c\u8102\u7cd6\u7c89\n  - 0.5\u00d7TBE\u62161\u00d7TAE\u7f13\u51b2\u6db2\n  - \u6eb4\u5316\u4e59\u952d\uff08Ethidium Bromide, EB\uff09\n  - \u4e0a\u6837\u7f13\u51b2\u6db2\uff08loading buffer\uff09\uff1a0.25%\u6eb4\u915a\u5170\uff0c40%\u8517\u7cd6\n- **\u5668\u5177**: \n  - \u7535\u6cf3\u7cfb\u7edf\uff08\u7535\u6cf3\u4eea\u3001\u6c34\u5e73\u7535\u6cf3\u69fd\u3001\u5236\u80f6\u677f\uff09\n  - \u7d2b\u5916\u900f\u5c04\u4eea\n  - \u79fb\u6db2\u5668\u53ca\u5438\u5934\n  - \u9525\u5f62\u74f6\n\n#### \u64cd\u4f5c\u6b65\u9aa4\n\n1. **\u914d\u5236\u743c\u8102\u7cd6\u51dd\u80f6**:\n   - \u6839\u636e\u9700\u8981\u5206\u79bb\u7684DNA\u7247\u6bb5\u5927\u5c0f\uff0c\u79f0\u53d6\u9002\u91cf\u7684\u743c\u8102\u7cd6\u7c89\uff08\u4f8b\u59821g\uff09\u653e\u5165\u9525\u5f62\u74f6\u4e2d\u3002\n   - \u52a0\u5165\u9002\u91cf\u76840.5\u00d7TBE\u62161\u00d7TAE\u7f13\u51b2\u6db2\uff08\u4f8b\u5982100ml\uff09\u3002\n   - \u5c06\u9525\u5f62\u74f6\u653e\u5165\u5fae\u6ce2\u7089\u4e2d\u52a0\u70ed\u81f3\u743c\u8102\u7cd6\u5b8c\u5168\u6eb6\u89e3\uff0c\u6eb6\u6db2\u53d8\u5f97\u900f\u660e\u3002\n   - \u51b7\u5374\u743c\u8102\u7cd6\u6eb6\u6db2\u81f3\u7ea660\u2103\uff0c\u52a0\u5165\u6eb4\u5316\u4e59\u952d\u81f3\u7ec8\u6d53\u5ea60.5\u00b5g/ml\uff08\u907f\u514d\u5149\u7167\uff09\u3002\n   - \u5728\u6c34\u5e73\u7535\u6cf3\u69fd\u5185\u653e\u7f6e\u5236\u80f6\u677f\uff0c\u5bc6\u5c01\u56db\u5468\uff0c\u5e76\u63d2\u5165\u68b3\u5b50\u3002\n   - \u7f13\u6162\u5012\u5165\u743c\u8102\u7cd6\u6eb6\u6db2\uff0c\u907f\u514d\u6c14\u6ce1\u4ea7\u751f\uff0c\u9759\u7f6e30-40\u5206\u949f\u76f4\u81f3\u51dd\u56fa\u3002\n\n2. **\u51c6\u5907\u7535\u6cf3\u69fd**:\n   - \u5c0f\u5fc3\u5730\u62d4\u51fa\u68b3\u5b50\uff0c\u786e\u4fdd\u51dd\u80f6\u5b54\u5b8c\u6574\u3002\n   - \u5c06\u51dd\u56fa\u7684\u51dd\u80f6\u653e\u5165\u7535\u6cf3\u69fd\u4e2d\u3002\n   - \u52a0\u5165\u7535\u6cf3\u7f13\u51b2\u6db2\uff0c\u6db2\u9762\u5e94\u7a0d\u5fae\u8986\u76d6\u51dd\u80f6\u8868\u9762\u3002\n\n3. **\u4e0a\u6837**:\n   - \u5c06DNA\u6837\u54c1\u4e0e\u4e0a\u6837\u7f13\u51b2\u6db2\u6df7\u5408\uff08\u4f8b\u59825\u00b5l DNA\u6837\u54c1\u52a0\u51651\u00b5l loading buffer\uff09\u3002\n   - \u4f7f\u7528\u79fb\u6db2\u5668\u5c06\u6df7\u5408\u6db2\u5c0f\u5fc3\u5730\u52a0\u5165\u51dd\u80f6\u5b54\u4e2d\uff0c\u907f\u514d\u6c14\u6ce1\u4ea7\u751f\u3002\n\n4. **\u8fd0\u884c\u7535\u6cf3**:\n   - \u63a5\u901a\u7535\u6e90\uff0c\u8bbe\u5b9a\u7535\u538b\u4e3a40-50V\uff0c\u8fd0\u884c30-40\u5206\u949f\u3002\n   - \u786e\u4fdd\u7535\u6cf3\u7f13\u51b2\u6db2\u8986\u76d6\u51dd\u80f6\u8868\u9762\uff0c\u4ee5\u9632\u6b62\u51dd\u80f6\u5e72\u88c2\u3002\n\n5. **\u89c2\u5bdf\u548c\u8bb0\u5f55\u7ed3\u679c**:\n   - \u7535\u6cf3\u7ed3\u675f\u540e\uff0c\u5173\u95ed\u7535\u6e90\u3002\n   - \u5c06\u51dd\u80f6\u79fb\u81f3\u7d2b\u5916\u900f\u5c04\u4eea\u4e0a\u8fdb\u884c\u89c2\u5bdf\uff0c\u5e76\u62cd\u7167\u8bb0\u5f55DNA\u6761\u5e26\u4f4d\u7f6e\u548c\u5927\u5c0f\u3002\n   - \u6839\u636eMarker\u5224\u65adDNA\u7247\u6bb5\u7684\u5927\u5c0f\u3002\n\n#### \u6ce8\u610f\u4e8b\u9879\n- **\u7f13\u51b2\u6db2\u9009\u62e9**: \u5bf9\u4e8e\u5c0f\u7247\u6bb5DNA\uff08<1000 bp\uff09\uff0c\u4f7f\u75281\u00d7TBE\u7f13\u51b2\u6db2\u6548\u679c\u66f4\u597d\uff0c\u800c\u5bf9\u4e8e\u5927DNA\u7247\u6bb5\uff08>12 kb\uff09\uff0c\u5efa\u8bae\u4f7f\u75281\u00d7TAE\u7f13\u51b2\u6db2\u301011\u2020source\u3011\u301012\u2020source\u3011\u3002\n- **\u51dd\u80f6\u6d53\u5ea6\u9009\u62e9**: \u743c\u8102\u7cd6\u6d53\u5ea6\u7684\u9009\u62e9\u5e94\u6839\u636e\u5206\u79bb\u7684DNA\u5927\u5c0f\u8303\u56f4\u8c03\u6574\uff0c\u901a\u5e38\u4f7f\u75280.7%-2%\u7684\u743c\u8102\u7cd6\u6d53\u5ea6\u301013\u2020source\u3011\u301014\u2020source\u3011\u3002\n- **\u5b89\u5168\u9632\u62a4**: \u6eb4\u5316\u4e59\u952d\u5177\u6709\u6bd2\u6027\uff0c\u64cd\u4f5c\u65f6\u9700\u4f69\u6234\u624b\u5957\uff0c\u5e76\u5728\u901a\u98ce\u826f\u597d\u7684\u73af\u5883\u4e2d\u8fdb\u884c\uff0c\u5e9f\u5f03\u7269\u5904\u7406\u9700\u7b26\u5408\u5b89\u5168\u89c4\u5b9a\u301012\u2020source\u3011\u3002\n\n\u4ee5\u4e0a\u662f\u5b9e\u9a8c\u5ba4\u4e2d\u8fdb\u884cDNA\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u7684\u8be6\u7ec6\u64cd\u4f5c\u6b65\u9aa4\uff0c\u786e\u4fdd\u6bcf\u4e00\u6b65\u90fd\u4e25\u683c\u6309\u7167\u89c4\u8303\u6267\u884c\uff0c\u4ee5\u4fdd\u8bc1\u5b9e\u9a8c\u7ed3\u679c\u7684\u51c6\u786e\u6027\u548c\u53ef\u91cd\u590d\u6027\u3002", "model": "gpt-4o-all", "timestamp": 1716015232, "done": true}, "a513174a-2d5b-40b2-84e5-cc1b297ef4fc": {"parentId": "a25669df-a668-4daf-aa8e-f31a72396f8c", "id": "a513174a-2d5b-40b2-84e5-cc1b297ef4fc", "childrenIds": [], "role": "assistant", "content": "# \u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u64cd\u4f5c\u6b65\u9aa4\n\n\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u662f\u5b9e\u9a8c\u5ba4\u4e2d\u68c0\u6d4bDNA\u7684\u5e38\u7528\u65b9\u6cd5\u3002\u4ee5\u4e0b\u662f\u8be6\u7ec6\u7684\u64cd\u4f5c\u6b65\u9aa4:\n\n#### \u51c6\u5907\u5de5\u4f5c\n1. \u914d\u52361X TAE\u7f13\u51b2\u6db2(40mM Tris, 20mM \u4e59\u9178, 1mM EDTA, pH 8.0)\u3002\n2. \u79f0\u53d6\u9002\u91cf\u7684\u743c\u8102\u7cd6\u7c89\u672b,\u52a0\u51651X TAE\u7f13\u51b2\u6db2\u4e2d,\u52a0\u70ed\u6eb6\u89e3\u81f3\u6f84\u6e05\u900f\u660e,\u51b7\u5374\u81f3\u7ea660\u00b0C\u3002\n3. \u5728\u7535\u6cf3\u69fd\u4e24\u7aef\u5404\u653e\u4e00\u5757\u80f6\u57ab,\u4e2d\u95f4\u653e\u4e00\u5757\u73bb\u7483\u677f,\u7528\u5939\u5b50\u56fa\u5b9a\u3002\n4. \u5c0660\u00b0C\u5de6\u53f3\u7684\u743c\u8102\u7cd6\u6eb6\u6db2\u5012\u5165\u7535\u6cf3\u69fd\u4e2d,\u63d2\u5165\u68b3\u5b50\u5f62\u6210\u6837\u54c1\u5b54,\u9759\u7f6e\u81f3\u5b8c\u5168\u51dd\u56fa(\u7ea630\u5206\u949f)\u3002\n\n#### \u4e0a\u6837\u4e0e\u7535\u6cf3\n1. \u53d6\u51fa\u68b3\u5b50,\u5c06\u51dd\u80f6\u8fde\u540c\u73bb\u7483\u677f\u4e00\u8d77\u653e\u5165\u7535\u6cf3\u69fd,1X TAE\u7f13\u51b2\u6db2\u6ca1\u8fc7\u51dd\u80f6\u8868\u9762\u3002\n2. \u53d6\u9002\u91cfDNA\u6837\u54c1\u4e0e6X\u4e0a\u6837\u7f13\u51b2\u6db2\u6df7\u5300,\u5c0f\u5fc3\u52a0\u5165\u6837\u54c1\u5b54\u4e2d\u3002\n3. \u76d6\u4e0a\u7535\u6cf3\u4eea\u76d6\u5b50,\u63a5\u901a\u7535\u6e90,\u6839\u636e\u51dd\u80f6\u5927\u5c0f\u548c\u6d53\u5ea6\u8bbe\u7f6e\u5408\u9002\u7684\u7535\u538b(\u901a\u5e38\u4e3a5V/cm),\u7535\u6cf330-40\u5206\u949f\u3002\n\n#### \u67d3\u8272\u4e0e\u89c2\u5bdf\n1. \u5173\u95ed\u7535\u6e90,\u53d6\u51fa\u51dd\u80f6,\u653e\u5165\u542b\u6709\u6eb4\u5316\u4e59\u952d(EB)\u7684\u67d3\u8272\u7f13\u51b2\u6db2\u4e2d,\u907f\u5149\u67d3\u827210-30\u5206\u949f\u3002\n2. \u53d6\u51fa\u51dd\u80f6,\u7528\u6c34\u51b2\u6d17\u6389\u8868\u9762\u7684EB,\u653e\u5728\u51dd\u80f6\u6210\u50cf\u4eea\u4e0a\u62cd\u7167\u3002\n3. \u5728\u7d2b\u5916\u706f\u4e0b\u89c2\u5bdf\u6761\u5e26,\u786e\u5b9a\u76ee\u7684DNA\u7247\u6bb5\u7684\u5927\u5c0f\u3002\n\n#### \u6ce8\u610f\u4e8b\u9879\n- \u914d\u5236\u548c\u4f7f\u7528EB\u65f6\u8981\u6234\u624b\u5957,\u907f\u514d\u63a5\u89e6\u76ae\u80a4\u3002\n- \u7535\u6cf3\u65f6\u6837\u54c1\u8981\u52a0\u5230\u51dd\u80f6\u5b54\u7684\u5e95\u90e8,\u907f\u514d\u6269\u6563\u3002\n- \u67d3\u8272\u65f6\u95f4\u8fc7\u957f\u4f1a\u5bfc\u81f4\u80cc\u666f\u67d3\u8272\u52a0\u6df1,\u4e0d\u6613\u5224\u8bfb\u7ed3\u679c\u3002\n- \u7d2b\u5916\u706f\u4e0b\u89c2\u5bdf\u548c\u62cd\u7167DNA\u6761\u5e26\u65f6\u8981\u5c3d\u91cf\u7f29\u77ed\u65f6\u95f4,\u907f\u514dDNA\u635f\u4f24\u3002\n\n\u901a\u8fc7\u89c4\u8303\u64cd\u4f5c\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3,\u53ef\u4ee5\u51c6\u786e\u5206\u79bb\u4e0d\u540c\u5927\u5c0f\u7684DNA\u7247\u6bb5,\u4e3a\u540e\u7eed\u7684\u5206\u5b50\u514b\u9686\u548c\u6d4b\u5e8f\u5b9e\u9a8c\u5960\u5b9a\u57fa\u7840\u3002", "model": "claude-3-opus-20240229", "timestamp": 1716015232, "done": true}}, "currentId": "dd76f84f-e67b-49e8-b970-3b00e408d62a"}, "tags": [], "timestamp": 1716003393587}


id: bcb74859-1e24-4c02-858e-2db00a010a23
user_id: 05d069f6-4441-41d7-bbee-ac0277857a6c
title: 模型型号查询
chat:
  {"id": "", "title": "\u6a21\u578b\u578b\u53f7\u67e5\u8be2", "models": ["qwen-max"], "system": "\u4f60\u662f\u4e00\u4e2a\u6709\u7528\u7684\u52a9\u624b", "options": {}, "messages": [{"id": "cfb3818c-ad0e-4a0f-bf2b-37c5f610107c", "parentId": null, "childrenIds": ["d502336a-a6eb-4c73-aa8f-f010bc9ab51b", "1d27316b-a62d-4430-945b-a5139f3dac59", "ae64f5d2-42d8-4041-afcb-1f679dd09a39", "e9c3de3e-8e9e-4c06-bda9-a3f56b1b7c3c"], "role": "user", "content": "\u4f60\u7684\u6a21\u578b\u578b\u53f7\u662f\u4ec0\u4e48", "timestamp": 1716030892}, {"parentId": "cfb3818c-ad0e-4a0f-bf2b-37c5f610107c", "id": "e9c3de3e-8e9e-4c06-bda9-a3f56b1b7c3c", "childrenIds": [], "role": "assistant", "content": "\u6211\u662f\u6765\u81ea\u963f\u91cc\u4e91\u7684\u8d85\u5927\u89c4\u6a21\u8bed\u8a00\u6a21\u578b\uff0c\u6211\u53eb\u901a\u4e49\u5343\u95ee\u3002", "model": "qwen-plus", "timestamp": 1716031050, "done": true}], "history": {"messages": {"cfb3818c-ad0e-4a0f-bf2b-37c5f610107c": {"id": "cfb3818c-ad0e-4a0f-bf2b-37c5f610107c", "parentId": null, "childrenIds": ["d502336a-a6eb-4c73-aa8f-f010bc9ab51b", "1d27316b-a62d-4430-945b-a5139f3dac59", "ae64f5d2-42d8-4041-afcb-1f679dd09a39", "e9c3de3e-8e9e-4c06-bda9-a3f56b1b7c3c"], "role": "user", "content": "\u4f60\u7684\u6a21\u578b\u578b\u53f7\u662f\u4ec0\u4e48", "timestamp": 1716030892}, "d502336a-a6eb-4c73-aa8f-f010bc9ab51b": {"parentId": "cfb3818c-ad0e-4a0f-bf2b-37c5f610107c", "id": "d502336a-a6eb-4c73-aa8f-f010bc9ab51b", "childrenIds": [], "role": "assistant", "content": "", "model": "qwen-max", "timestamp": 1716030893, "done": true}, "1d27316b-a62d-4430-945b-a5139f3dac59": {"parentId": "cfb3818c-ad0e-4a0f-bf2b-37c5f610107c", "id": "1d27316b-a62d-4430-945b-a5139f3dac59", "childrenIds": [], "role": "assistant", "content": "", "model": "qwen-max", "timestamp": 1716030999, "done": true}, "ae64f5d2-42d8-4041-afcb-1f679dd09a39": {"parentId": "cfb3818c-ad0e-4a0f-bf2b-37c5f610107c", "id": "ae64f5d2-42d8-4041-afcb-1f679dd09a39", "childrenIds": [], "role": "assistant", "content": "", "model": "qwen2.5", "timestamp": 1716031026, "done": true}, "e9c3de3e-8e9e-4c06-bda9-a3f56b1b7c3c": {"parentId": "cfb3818c-ad0e-4a0f-bf2b-37c5f610107c", "id": "e9c3de3e-8e9e-4c06-bda9-a3f56b1b7c3c", "childrenIds": [], "role": "assistant", "content": "\u6211\u662f\u6765\u81ea\u963f\u91cc\u4e91\u7684\u8d85\u5927\u89c4\u6a21\u8bed\u8a00\u6a21\u578b\uff0c\u6211\u53eb\u901a\u4e49\u5343\u95ee\u3002", "model": "qwen-plus", "timestamp": 1716031050, "done": true}}, "currentId": "e9c3de3e-8e9e-4c06-bda9-a3f56b1b7c3c"}, "tags": [], "timestamp": 1716030892566}


id: 881c0f75-412c-45b2-8032-5d848748d6e2
user_id: 05d069f6-4441-41d7-bbee-ac0277857a6c
title:
  For query what type of model are you, create a concise title consisting of 3-5 words without using the word title within the limit of 3-5 words: Model Type.

chat:
  {"id": "", "title": "\nFor query what type of model are you, create a concise title consisting of 3-5 words without using the word title within the limit of 3-5 words: Model Type.", "models": ["research"], "system": "\u4f60\u662f\u4e00\u4e2a\u6709\u7528\u7684\u52a9\u624b", "options": {}, "messages": [{"id": "916c72fa-4fea-4e1d-9772-d464f56e54c4", "parentId": null, "childrenIds": ["7fb7e331-5bda-429a-b023-56041fecffd0", "6cc92055-31b7-405e-985f-714f9fecbfdd", "df77b97a-a69d-43e6-8dad-a8fa6160f616", "673caefb-4eda-4c0b-bc53-3f066e1b98b1", "27961cc4-58ff-4770-9135-f3b02827741d"], "role": "user", "content": "\u4f60\u662f\u4ec0\u4e48\u6a21\u578b", "timestamp": 1716179850}, {"parentId": "916c72fa-4fea-4e1d-9772-d464f56e54c4", "id": "27961cc4-58ff-4770-9135-f3b02827741d", "childrenIds": [], "role": "assistant", "content": "Based on the provided API responses, it appears that there is no straightforward solution to ChatGPT from responding to irrelevant questions beyond ensuring that the initial instructions (or system prompts) accurately reflect the desired context. Some developers have reported success in controlling ChatGPT's responses by narrowing down the problem and defining specific contexts for each question. However, doing so requires additional effort and may still result in some unwanted responses. Additionally, some developers have suggested extending the API to create custom endpoints or modifying existing ones to retrieve user roles, but these solutions require further investigation. Overall, while ChatGPT can be helpful for providing answers to factual questions, its limitations in handling complex queries and maintaining consistent contexts should be considered. Users should approach its responses with caution and cross-reference them with reliable sources where necessary.", "model": "zephyr", "timestamp": 1716560931, "done": true}], "history": {"messages": {"916c72fa-4fea-4e1d-9772-d464f56e54c4": {"id": "916c72fa-4fea-4e1d-9772-d464f56e54c4", "parentId": null, "childrenIds": ["7fb7e331-5bda-429a-b023-56041fecffd0", "6cc92055-31b7-405e-985f-714f9fecbfdd", "df77b97a-a69d-43e6-8dad-a8fa6160f616", "673caefb-4eda-4c0b-bc53-3f066e1b98b1", "27961cc4-58ff-4770-9135-f3b02827741d"], "role": "user", "content": "\u4f60\u662f\u4ec0\u4e48\u6a21\u578b", "timestamp": 1716179850}, "7fb7e331-5bda-429a-b023-56041fecffd0": {"parentId": "916c72fa-4fea-4e1d-9772-d464f56e54c4", "id": "7fb7e331-5bda-429a-b023-56041fecffd0", "childrenIds": [], "role": "assistant", "content": "\u79d8\u5854AI\u641c\u7d22\u91c7\u7528\u7684MetaLLM\u6a21\u578b\u7531\u79d8\u5854\u79d1\u6280\u4e8e2022\u5e74\u5168\u6808\u81ea\u7814\u3002\u8be5\u6a21\u578b\u4e8e2023\u5e74\u901a\u8fc7\u300a\u751f\u6210\u5f0f\u5927\u6a21\u578b\u5907\u6848\u300b\uff08\u7b2c\u4e8c\u6279\uff09\u3001\u300a\u5927\u6a21\u578b\u7b97\u6cd5\u5907\u6848\u300b\uff08\u7b2c\u4e8c\u6279\uff09\u3002\n\u76ee\u524d\u79d8\u5854AI\u641c\u7d22\u5c1a\u5904\u4e8e0.99\u7248\u7814\u53d1\u6d4b\u8bd5\u9636\u6bb5\uff0c\u6211\u4eec\u6df1\u77e5\u5f53\u524d\u6a21\u578b\u53ca\u4ea7\u54c1\u6548\u679c\u8fd8\u5b58\u5728\u8bf8\u591a\u53ef\u63d0\u5347\u7a7a\u95f4\uff0c\u8fd8\u5e0c\u671b\u5927\u5bb6\u7ed9\u6211\u4eec\u591a\u4e00\u4e9b\u8010\u5fc3\uff0c\u8ba9\u6211\u4eec\u5728\u6709\u9650\u8d44\u6e90\u4e4b\u4e0b\u6301\u7eed\u63d0\u5347\u4ea7\u54c1\u3002", "model": "research", "timestamp": 1716179851, "done": true}, "6cc92055-31b7-405e-985f-714f9fecbfdd": {"parentId": "916c72fa-4fea-4e1d-9772-d464f56e54c4", "id": "6cc92055-31b7-405e-985f-714f9fecbfdd", "childrenIds": [], "role": "assistant", "content": "\u54ce\u5440\uff01\u8fde\u63a5\u5230detail\u65f6\u51fa\u73b0\u95ee\u9898\u3002", "model": "detail", "timestamp": 1716560898, "error": true, "done": true}, "df77b97a-a69d-43e6-8dad-a8fa6160f616": {"parentId": "916c72fa-4fea-4e1d-9772-d464f56e54c4", "id": "df77b97a-a69d-43e6-8dad-a8fa6160f616", "childrenIds": [], "role": "assistant", "content": "\u54ce\u5440\uff01\u8fde\u63a5\u5230concise\u65f6\u51fa\u73b0\u95ee\u9898\u3002", "model": "concise", "timestamp": 1716560904, "error": true, "done": true}, "673caefb-4eda-4c0b-bc53-3f066e1b98b1": {"parentId": "916c72fa-4fea-4e1d-9772-d464f56e54c4", "id": "673caefb-4eda-4c0b-bc53-3f066e1b98b1", "childrenIds": [], "role": "assistant", "content": "\u54ce\u5440\uff01\u8fde\u63a5\u5230emohaa\u65f6\u51fa\u73b0\u95ee\u9898\u3002", "model": "emohaa", "timestamp": 1716560915, "error": true, "done": true}, "27961cc4-58ff-4770-9135-f3b02827741d": {"parentId": "916c72fa-4fea-4e1d-9772-d464f56e54c4", "id": "27961cc4-58ff-4770-9135-f3b02827741d", "childrenIds": [], "role": "assistant", "content": "Based on the provided API responses, it appears that there is no straightforward solution to ChatGPT from responding to irrelevant questions beyond ensuring that the initial instructions (or system prompts) accurately reflect the desired context. Some developers have reported success in controlling ChatGPT's responses by narrowing down the problem and defining specific contexts for each question. However, doing so requires additional effort and may still result in some unwanted responses. Additionally, some developers have suggested extending the API to create custom endpoints or modifying existing ones to retrieve user roles, but these solutions require further investigation. Overall, while ChatGPT can be helpful for providing answers to factual questions, its limitations in handling complex queries and maintaining consistent contexts should be considered. Users should approach its responses with caution and cross-reference them with reliable sources where necessary.", "model": "zephyr", "timestamp": 1716560931, "done": true}}, "currentId": "27961cc4-58ff-4770-9135-f3b02827741d"}, "tags": [], "timestamp": 1716179850656}


id: 6eb49ca1-5c97-4dd6-a211-a1f8c481ad5a
user_id: 05d069f6-4441-41d7-bbee-ac0277857a6c
title: 四边形和三角形面积比
chat:
  {"id": "", "title": "\u56db\u8fb9\u5f62\u548c\u4e09\u89d2\u5f62\u9762\u79ef\u6bd4", "models": ["gpt-4", "claude-3-opus-20240229", "gpt-4-o"], "system": "\u4f60\u662f\u4e00\u4e2a\u6709\u7528\u7684\u52a9\u624b", "options": {}, "messages": [{"id": "e6cf0b5d-5644-40f4-9923-1a4b8d436ba2", "parentId": null, "childrenIds": ["71d0087f-b2d2-44f0-9b7f-d5f5986750dd", "b9bd0562-b472-45d8-8afb-d90fce2933e2", "6c73930a-63ef-466f-8699-3445ce680223"], "role": "user", "content": "\u8bf7\u5c1d\u8bd5\u89e3\u51b3\uff1a14. \u5728\u56db\u8fb9\u5f62 \\(ABCD\\) \u4e2d\uff0c\\(\\overrightarrow{BC} = 2 \\overrightarrow{AD}\\)\uff0c\u70b9 \\(P\\) \u662f\u56db\u8fb9\u5f62 \\(ABCD\\) \u6240\u5728\u5e73\u9762\u4e0a\u4e00\u70b9\uff0c\u6ee1\u8db3 \\(\\overrightarrow{PA} + 10 \\overrightarrow{PB} + \\overrightarrow{PC} + 10 \\overrightarrow{PD} = 0\\)\u3002\u8bbe \\(s, t\\) \u5206\u522b\u4e3a\u56db\u8fb9\u5f62 \\(ABCD\\) \u4e0e \\(\\triangle PAB\\) \u7684\u9762\u79ef\uff0c\u5219 \\(\\frac{t}{s} = \\underline{\\hspace{2cm}}\\)\u3002", "timestamp": 1716196101}, {"parentId": "e6cf0b5d-5644-40f4-9923-1a4b8d436ba2", "id": "71d0087f-b2d2-44f0-9b7f-d5f5986750dd", "childrenIds": [], "role": "assistant", "content": "\u9996\u5148\uff0c\u6ce8\u610f\u5230\u5411\u91cf \\(\\overrightarrow{BC}\\) \u548c \\(\\overrightarrow{AD}\\) \u6070\u597d\u662f\u56db\u8fb9\u5f62 \\(ABCD\\) \u7684\u4e00\u7ec4\u5bf9\u89d2\u7ebf\u5411\u91cf\uff0c\u5e76\u4e14\u8fd9\u4e24\u4e2a\u5411\u91cf\u7684\u5173\u7cfb\u662f \\(\\overrightarrow{BC} = 2 \\overrightarrow{AD}\\)\u3002\u540c\u65f6\uff0c\u8fd9\u4e24\u4e2a\u5411\u91cf\u4e5f\u6070\u597d\u662f\u56db\u8fb9\u5f62 \\(ABCD\\) \u6240\u5728\u5e73\u9762\u7684\u4e00\u7ec4\u57fa\u5411\u91cf\u3002\n\n\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u5c06 \\(\\overrightarrow{PA} + 10 \\overrightarrow{PB} + \\overrightarrow{PC} + 10 \\overrightarrow{PD} = 0\\) \u6539\u5199\u4e3a\uff1a\n\\(\\overrightarrow{PA} + \\overrightarrow{PC} = 10 \\overrightarrow{PB} + 10 \\overrightarrow{PD}\\)\n\n\u5c06\u5176\u7406\u89e3\u4e3a\u56db\u8fb9\u5f62 \\(ABCD\\) \u6240\u5728\u5e73\u9762\u7684\u4e24\u7ec4\u5411\u91cf\u4e4b\u95f4\u7684\u5173\u7cfb\u3002\u6211\u4eec\u770b\u51fa\uff0c\u8fd9\u4e2a\u5173\u7cfb\u544a\u8bc9\u6211\u4eec\uff0c\u8d28\u5fc3G\u5904\u7684\u67d0\u4e00\u70b9\u5411\u91cf\u7b49\u4e8eP\u5904\u7684\u67d0\u4e00\u70b9\u5411\u91cf\u3002\u7531\u6b64\u6211\u4eec\u63a8\u51fa\uff0cP\u5fc5\u987b\u7b49\u4e8eG\u70b9\u3002\n\n\u56e0\u4e3a\u70b9 \\(P\\) \u5c31\u662f\u56db\u8fb9\u5f62 \\(ABCD\\) \u7684\u8d28\u5fc3\uff0c\u6240\u4ee5\u5b83\u5c06\u56db\u8fb9\u5f62 \\(ABCD\\) \u5212\u5206\u4e3a\u56db\u4e2a\u5c0f\u7684\u540c\u9762\u79ef\u4e09\u89d2\u5f62\uff0c\u5373 \\(s = 4t\\)\u3002\n\n\u6240\u4ee5\uff0c\u63d2\u5165\u6b64\u7ed3\u679c\uff0c\u7136\u540e\u5f97\u5230\u6bd4\u4f8b \\(\\frac{t}{s} = \\frac{1}{4}\\)\u3002\n", "model": "gpt-4", "timestamp": 1716196102, "done": true}], "history": {"messages": {"e6cf0b5d-5644-40f4-9923-1a4b8d436ba2": {"id": "e6cf0b5d-5644-40f4-9923-1a4b8d436ba2", "parentId": null, "childrenIds": ["71d0087f-b2d2-44f0-9b7f-d5f5986750dd", "b9bd0562-b472-45d8-8afb-d90fce2933e2", "6c73930a-63ef-466f-8699-3445ce680223"], "role": "user", "content": "\u8bf7\u5c1d\u8bd5\u89e3\u51b3\uff1a14. \u5728\u56db\u8fb9\u5f62 \\(ABCD\\) \u4e2d\uff0c\\(\\overrightarrow{BC} = 2 \\overrightarrow{AD}\\)\uff0c\u70b9 \\(P\\) \u662f\u56db\u8fb9\u5f62 \\(ABCD\\) \u6240\u5728\u5e73\u9762\u4e0a\u4e00\u70b9\uff0c\u6ee1\u8db3 \\(\\overrightarrow{PA} + 10 \\overrightarrow{PB} + \\overrightarrow{PC} + 10 \\overrightarrow{PD} = 0\\)\u3002\u8bbe \\(s, t\\) \u5206\u522b\u4e3a\u56db\u8fb9\u5f62 \\(ABCD\\) \u4e0e \\(\\triangle PAB\\) \u7684\u9762\u79ef\uff0c\u5219 \\(\\frac{t}{s} = \\underline{\\hspace{2cm}}\\)\u3002", "timestamp": 1716196101}, "71d0087f-b2d2-44f0-9b7f-d5f5986750dd": {"parentId": "e6cf0b5d-5644-40f4-9923-1a4b8d436ba2", "id": "71d0087f-b2d2-44f0-9b7f-d5f5986750dd", "childrenIds": [], "role": "assistant", "content": "\u9996\u5148\uff0c\u6ce8\u610f\u5230\u5411\u91cf \\(\\overrightarrow{BC}\\) \u548c \\(\\overrightarrow{AD}\\) \u6070\u597d\u662f\u56db\u8fb9\u5f62 \\(ABCD\\) \u7684\u4e00\u7ec4\u5bf9\u89d2\u7ebf\u5411\u91cf\uff0c\u5e76\u4e14\u8fd9\u4e24\u4e2a\u5411\u91cf\u7684\u5173\u7cfb\u662f \\(\\overrightarrow{BC} = 2 \\overrightarrow{AD}\\)\u3002\u540c\u65f6\uff0c\u8fd9\u4e24\u4e2a\u5411\u91cf\u4e5f\u6070\u597d\u662f\u56db\u8fb9\u5f62 \\(ABCD\\) \u6240\u5728\u5e73\u9762\u7684\u4e00\u7ec4\u57fa\u5411\u91cf\u3002\n\n\u63a5\u4e0b\u6765\uff0c\u6211\u4eec\u5c06 \\(\\overrightarrow{PA} + 10 \\overrightarrow{PB} + \\overrightarrow{PC} + 10 \\overrightarrow{PD} = 0\\) \u6539\u5199\u4e3a\uff1a\n\\(\\overrightarrow{PA} + \\overrightarrow{PC} = 10 \\overrightarrow{PB} + 10 \\overrightarrow{PD}\\)\n\n\u5c06\u5176\u7406\u89e3\u4e3a\u56db\u8fb9\u5f62 \\(ABCD\\) \u6240\u5728\u5e73\u9762\u7684\u4e24\u7ec4\u5411\u91cf\u4e4b\u95f4\u7684\u5173\u7cfb\u3002\u6211\u4eec\u770b\u51fa\uff0c\u8fd9\u4e2a\u5173\u7cfb\u544a\u8bc9\u6211\u4eec\uff0c\u8d28\u5fc3G\u5904\u7684\u67d0\u4e00\u70b9\u5411\u91cf\u7b49\u4e8eP\u5904\u7684\u67d0\u4e00\u70b9\u5411\u91cf\u3002\u7531\u6b64\u6211\u4eec\u63a8\u51fa\uff0cP\u5fc5\u987b\u7b49\u4e8eG\u70b9\u3002\n\n\u56e0\u4e3a\u70b9 \\(P\\) \u5c31\u662f\u56db\u8fb9\u5f62 \\(ABCD\\) \u7684\u8d28\u5fc3\uff0c\u6240\u4ee5\u5b83\u5c06\u56db\u8fb9\u5f62 \\(ABCD\\) \u5212\u5206\u4e3a\u56db\u4e2a\u5c0f\u7684\u540c\u9762\u79ef\u4e09\u89d2\u5f62\uff0c\u5373 \\(s = 4t\\)\u3002\n\n\u6240\u4ee5\uff0c\u63d2\u5165\u6b64\u7ed3\u679c\uff0c\u7136\u540e\u5f97\u5230\u6bd4\u4f8b \\(\\frac{t}{s} = \\frac{1}{4}\\)\u3002\n", "model": "gpt-4", "timestamp": 1716196102, "done": true}, "b9bd0562-b472-45d8-8afb-d90fce2933e2": {"parentId": "e6cf0b5d-5644-40f4-9923-1a4b8d436ba2", "id": "b9bd0562-b472-45d8-8afb-d90fce2933e2", "childrenIds": [], "role": "assistant", "content": "\u54ce\u5440\uff01\u8fde\u63a5\u5230claude-3-opus-20240229\u65f6\u51fa\u73b0\u95ee\u9898\u3002", "model": "claude-3-opus-20240229", "timestamp": 1716196102, "error": true, "done": true}, "6c73930a-63ef-466f-8699-3445ce680223": {"parentId": "e6cf0b5d-5644-40f4-9923-1a4b8d436ba2", "id": "6c73930a-63ef-466f-8699-3445ce680223", "childrenIds": [], "role": "assistant", "content": "\u6211\u4eec\u5c06\u5229\u7528\u5411\u91cf\u7684\u65b9\u6cd5\u89e3\u51b3\u8be5\u95ee\u9898\u3002\u9996\u5148\uff0c\u8bbe\u70b9 \\(A\\)\u3001\\(B\\)\u3001\\(C\\) \u548c \\(D\\) \u7684\u4f4d\u7f6e\u5411\u91cf\u5206\u522b\u4e3a \\(\\vec{a}\\)\u3001\\(\\vec{b}\\)\u3001\\(\\vec{c}\\) \u548c \\(\\vec{d}\\)\u3002\u7ed9\u5b9a\u6761\u4ef6\u662f \\(\\overrightarrow{BC} = 2 \\overrightarrow{AD}\\)\uff0c\u56e0\u6b64\u6211\u4eec\u6709\uff1a\n\\[\n\\vec{c} - \\vec{b} = 2 (\\vec{d} - \\vec{a})\n\\]\n\u5373\n\\[\n\\vec{c} = \\vec{b} + 2 (\\vec{d} - \\vec{a}) = 2 \\vec{d} - 2 \\vec{a} + \\vec{b}\n\\]\n\n\u6211\u4eec\u8fd8\u77e5\u9053\u70b9 \\(P\\) \u6ee1\u8db3 \\(\\overrightarrow{PA} + 10 \\overrightarrow{PB} + \\overrightarrow{PC} + 10 \\overrightarrow{PD} = 0\\)\u3002\u4f7f\u7528\u4f4d\u7f6e\u5411\u91cf\u8868\u793a\uff0c\u6211\u4eec\u6709\uff1a\n\\[\n(\\vec{p} - \\vec{a}) + 10 (\\vec{p} - \\vec{b}) + (\\vec{p} - \\vec{c}) + 10 (\\vec{p} - \\vec{d}) = 0\n\\]\n\u5c06\u6240\u6709 \\(\\vec{p}\\) \u9879\u5408\u5e76\u5728\u4e00\u8d77\uff1a\n\\[\n\\vec{p} - \\vec{a} + 10 \\vec{p} - 10 \\vec{b} + \\vec{p} - \\vec{c} + 10 \\vec{p} - 10 \\vec{d} = 0\n\\]\n\u5408\u5e76\u5f97\u5230\uff1a\n\\[\n22 \\vec{p} - (\\vec{a} + 10 \\vec{b} + \\vec{c} + 10 \\vec{d}) = 0\n\\]\n\u5373\n\\[\n22 \\vec{p} = \\vec{a} + 10 \\vec{b} + \\vec{c} + 10 \\vec{d}\n\\]\n\n\u63a5\u7740\u5c06 \\(\\vec{c}\\) \u7684\u8868\u8fbe\u5f0f\u4ee3\u5165\uff1a\n\\[\n22 \\vec{p} = \\vec{a} + 10 \\vec{b} + (2 \\vec{d} - 2 \\vec{a} + \\vec{b}) + 10 \\vec{d}\n\\]\n\\[\n22 \\vec{p} = \\vec{a} + 10 \\vec{b} + 2 \\vec{d} - 2 \\vec{a} + \\vec{b} + 10 \\vec{d}\n\\]\n\\[\n22 \\vec{p} = -\\vec{a} + 11 \\vec{b} + 12 \\vec{d}\n\\]\n\\[\n\\vec{p} = \\frac{-\\vec{a} + 11 \\vec{b} + 12 \\vec{d}}{22}\n\\]\n\n\u73b0\u5728\u6211\u4eec\u6765\u8ba1\u7b97\u56db\u8fb9\u5f62 \\(ABCD\\) \u4e0e\u4e09\u89d2\u5f62 \\(PAB\\) \u7684\u9762\u79ef\u6bd4\u503c\u3002\u9996\u5148\uff0c\u8bbe\u56db\u8fb9\u5f62 \\(ABCD\\) \u7684\u9762\u79ef\u4e3a \\(S\\)\u3002\u7531\u4e8e \\(\\overrightarrow{BC} = 2 \\overrightarrow{AD}\\)\uff0c\u610f\u5473\u7740 \\(ABCD\\) \u662f\u5e73\u884c\u56db\u8fb9\u5f62\uff0c\u4e14 \\(BC\\) \u662f \\(AD\\) \u76842\u500d\u3002\n\n\u4e3a\u4e86\u6c42\u51fa\u4e09\u89d2\u5f62 \\(PAB\\) \u7684\u9762\u79ef \\(t\\)\uff0c\u6211\u4eec\u9700\u8981\u6c42\u5f97\\(P\\)\u5230\\(AB\\)\u7684\u8ddd\u79bb\uff0c\u4ee5\u53ca\u4e09\u89d2\u5f62\u5e95\u8fb9 \\(AB\\) \u7684\u957f\u5ea6\u3002\u7531\u4e8e\u6211\u4eec\u4e0d\u80fd\u76f4\u63a5\u7528\u5411\u91cf\u6765\u8ba1\u7b97\u8fd9\u4e9b\uff0c\u6211\u4eec\u53ef\u4ee5\u501f\u52a9\u6bd4\u4f8b\u5173\u7cfb\uff1a\n\n\u8003\u8651\u5230 \\( \\vec{p} \\) \u662f\u6839\u636e \\(A, B, D\\) \u7684\u52a0\u6743\u5e73\u5747\u6240\u5f97\uff0c\u6211\u4eec\u5f97\u5230\uff1a\n\\[\n \\frac{t}{S} = \\frac{1}{22}\n\\]\n\u56e0\u4e3a\u5e73\u884c\u56db\u8fb9\u5f62\u88ab \\(P\\) \u5206\u4e3a22\u4e2a\u76f8\u7b49\u90e8\u5206\uff0c\u56e0\u6b64\n\\[\n\\frac{t}{S} = \\frac{1}{22}\n\\]\n\n\u6240\u4ee5\uff0c\u7b54\u6848\u4e3a\uff1a\n\\[\n\\boxed{\\frac{1}{22}}\n\\]", "model": "gpt-4-o", "timestamp": 1716196102, "done": true}}, "currentId": "71d0087f-b2d2-44f0-9b7f-d5f5986750dd"}, "tags": [], "timestamp": 1716196101673}


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title: 新聊天
chat:
  {"id": "", "title": "\u65b0\u804a\u5929", "models": ["gpt-4"], "options": {}, "messages": [{"id": "49373b50-0425-4f5b-8d1d-16f843842d02", "parentId": null, "childrenIds": ["d012c3ba-9be4-4e70-9929-e6e133f5db37"], "role": "user", "content": "Check the following sentence for grammar and clarity: \"[sentence]\". Rewrite it for better readability while maintaining its original meaning.", "timestamp": 1716560666}, {"parentId": "49373b50-0425-4f5b-8d1d-16f843842d02", "id": "d012c3ba-9be4-4e70-9929-e6e133f5db37", "childrenIds": [], "role": "assistant", "content": "I'm sorry, but there seems to be a misunderstanding. The sentence you want me to check for grammar and clarity is not provided. Could you please provide the sentence you want me to check?", "model": "gpt-4", "timestamp": 1716560667, "done": true}], "history": {"messages": {"49373b50-0425-4f5b-8d1d-16f843842d02": {"id": "49373b50-0425-4f5b-8d1d-16f843842d02", "parentId": null, "childrenIds": ["d012c3ba-9be4-4e70-9929-e6e133f5db37"], "role": "user", "content": "Check the following sentence for grammar and clarity: \"[sentence]\". Rewrite it for better readability while maintaining its original meaning.", "timestamp": 1716560666}, "d012c3ba-9be4-4e70-9929-e6e133f5db37": {"parentId": "49373b50-0425-4f5b-8d1d-16f843842d02", "id": "d012c3ba-9be4-4e70-9929-e6e133f5db37", "childrenIds": [], "role": "assistant", "content": "I'm sorry, but there seems to be a misunderstanding. The sentence you want me to check for grammar and clarity is not provided. Could you please provide the sentence you want me to check?", "model": "gpt-4", "timestamp": 1716560667, "done": true}}, "currentId": "d012c3ba-9be4-4e70-9929-e6e133f5db37"}, "tags": [], "timestamp": 1716560666464}


# user

id: 05d069f6-4441-41d7-bbee-ac0277857a6c
name: Jerry Zhou
email: i@jerryz.com.cn
role: admin
profile_image_url:
  https://www.gravatar.com/avatar/8f4893d873cefb9951f119bbde2af10ce57c9749763cd318e1966480cb7addc5?d=mp


